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Titolo:
Separation of carvedilol enantiomers in very small volumes of human plasmaby capillary electrophoresis with laser-induced fluorescence
Autore:
Behn, F; Michels, S; Laer, S; Blaschke, G;
Indirizzi:
Univ Munster, Inst Pharmazeut Chem, D-48149 Munster, Germany Univ MunsterMunster Germany D-48149 zeut Chem, D-48149 Munster, Germany Univ Hamburg, Hosp Eppendorf, Inst Expt & Klin Pharmakol & Toxikol, Abt Pharmakol, D-20246 Hamburg, Germany Univ Hamburg Hamburg Germany D-20246 Pharmakol, D-20246 Hamburg, Germany
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 755, anno: 2001,
pagine: 111 - 117
SICI:
1387-2273(20010505)755:1-2<111:SOCEIV>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEART-FAILURE; PHARMACOKINETICS; VALIDATION;
Keywords:
enantiomer separation; carvedilol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Blaschke, G Univ Munster, Inst Pharmazeut Chem, Hittorfstr 58-62, D-48149 Munster, Germany Univ Munster Hittorfstr 58-62 Munster Germany D-48149 Germany
Citazione:
F. Behn et al., "Separation of carvedilol enantiomers in very small volumes of human plasmaby capillary electrophoresis with laser-induced fluorescence", J CHROMAT B, 755(1-2), 2001, pp. 111-117

Abstract

A sensitive capillary electrophoretic method for the determination of carvedilol enantiomers in 100 mul of human plasma has been developed and validated. Carvedilol and the internal standard carazolol are isolated from plasma samples by Liquid-liquid extraction using diethylether. A sensitive and selective detection is provided by helium-cadmium laser-induced fluorescence. The total analysis time is 17.5 min, about 30 min are needed for the sample preparation. The linearity of the assay ranges from 1.56 to 50 ng/ml percarvedilol enantiomer. The limits of quantification (LOQ) for the carvedilol enantiomers in 100 yl of human plasma are 1.56 ng/ml. The inter-day accuracy for R-carvedilol is between 95.8 and 103% (104% at LOQ) and for S-carvedilol between 97.1 and 103% (107% at LOQ); the inter-day precision values are between 3.81 and 8.64% (10.9% at LOQ) and between 5.47 and 7.86% (7.91%at LOQ) for R- and S-carvedilol, respectively. The small sample volume needed is especially advantageous for the application in clinical studies in pediatric patients. As an application of the assay concentration/time profiles of the carvedilol enantiomers in a 5-year-old patient receiving a test dose of 0.09 mg/kg carvedilol are reported. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:34:33