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Titolo:
Use of non-covalent labeling in illustrating ligand binding to human serumalbumin via affinity capillary electrophoresis with near-infrared laser induced fluorescence detection
Autore:
Sowell, J; Agnew-Heard, KA; Mason, JC; Mama, C; Strekowski, L; Patonay, G;
Indirizzi:
Georgia State Univ, Dept Chem, Atlanta, GA 30303 USA Georgia State Univ Atlanta GA USA 30303 Dept Chem, Atlanta, GA 30303 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 755, anno: 2001,
pagine: 91 - 99
SICI:
1387-2273(20010505)755:1-2<91:UONLII>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIODE-LASER; PROTEINS; ENANTIOMERS; SEPARATIONS; COMPLEXES; PROBE; DRUGS;
Keywords:
non-covalent labeling; human serum albumin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Patonay, G Georgia State Univ, Dept Chem, Univ Plaza, Atlanta, GA 30303 USA Georgia State Univ Univ Plaza Atlanta GA USA 30303 GA 30303 USA
Citazione:
J. Sowell et al., "Use of non-covalent labeling in illustrating ligand binding to human serumalbumin via affinity capillary electrophoresis with near-infrared laser induced fluorescence detection", J CHROMAT B, 755(1-2), 2001, pp. 91-99

Abstract

This paper demonstrates the use of a near-infrared (NIR) dye as a non-covalent label for human serum albumin (HSA). The dye is a water soluble, heptamethine cyanine dye. The utility of the dye as a tracer illustrating the binding of various drugs to HSA is demonstrated via affinity capillary electrophoresis with near-infrared laser-induced fluorescence detection (ACE-NIR-LIF). Additionally, the factors affecting the separation of relevant species were investigated. The change in quantum yield of the dye upon complexation with HSA was calculated. Spectrophotometric measurements were conducted to study the stoichiometry of the dye albumin complex. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 10:56:48