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Titolo:
Simultaneous determination of the HIV protease inhibitors indinavir, amprenavir, saquinavir, ritonavir and nelfinavir in human plasma by high-performance liquid chromatography
Autore:
Yamada, H; Kotaki, H; Nakamura, T; Iwamoto, A;
Indirizzi:
Univ Tokyo, Inst Med Sci, Res Hosp, Dept Pharm,Minato Ku, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 ept Pharm,Minato Ku, Tokyo 1088639, Japan Univ Tokyo, Inst Med Sci, Dept Infect Dis & Appl Immunol, Minato Ku, Tokyo1088639, Japan Univ Tokyo Tokyo Japan 1088639 l Immunol, Minato Ku, Tokyo1088639, Japan Univ Tokyo, Inst Med Sci, Adv Clin Res Ctr, Div Infect Dis,Minato Ku, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 nfect Dis,Minato Ku, Tokyo 1088639, Japan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 755, anno: 2001,
pagine: 85 - 89
SICI:
1387-2273(20010505)755:1-2<85:SDOTHP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ULTRAVIOLET DETECTION;
Keywords:
indinavir; amprenavir; saquinavir; ritonavir; nelfinavir;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
8
Recensione:
Indirizzi per estratti:
Indirizzo: Yamada, H Univ Tokyo, Inst Med Sci, Res Hosp, Dept Pharm,Minato Ku, 4-6-1 Shirokanedai, Tokyo 1088639, Japan Univ Tokyo 4-6-1 Shirokanedai Tokyo Japan 1088639 088639, Japan
Citazione:
H. Yamada et al., "Simultaneous determination of the HIV protease inhibitors indinavir, amprenavir, saquinavir, ritonavir and nelfinavir in human plasma by high-performance liquid chromatography", J CHROMAT B, 755(1-2), 2001, pp. 85-89

Abstract

A reversed-phase high-performance liquid chromatographic method for the simultaneous quantitative determination of five HIV protease inhibitors (i.e.indinavir, amprenavir, saquinavir, ritonavir and nelfinavir) in human plasma is described. An aliquot of 500 mul plasma was extracted with 0.5 mi of 0.1 M NH4OH and 5 mi of methyl tert.-butyl ether. After evaporating, the residue was dissolved in eluent mixture of acetonitrile and 50 mM KH2PO4 adjusted to pH 5.6 with 50 mM Na2HPO4 (43:57, v/v). Subsequently, the eluent was washed with hexane. Chromatography was performed using a C-18 reversed-phase column. Ultraviolet detection at 215 nm was used. Linearity of the method was obtained in the concentration range of 0.05-20 mug ml(-1) for all five protease inhibitors. Our method is now in use to analyse plasma samples from patients treated with co-administration of HIV protease inhibitors. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:16:34