Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Automated sample preparation and gas chromatographic-mass spectrometric analysis of urinary androgenic anabolic steroids
Autore:
Haber, E; Munoz-Guerra, JA; Soriano, C; Carreras, D; Rodriguez, C; Rodriguez, FA;
Indirizzi:
Higher Sports Council, Doping Control Lab, Madrid 28040, Spain Higher Sports Council Madrid Spain 28040 ontrol Lab, Madrid 28040, Spain
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 755, anno: 2001,
pagine: 17 - 26
SICI:
1387-2273(20010505)755:1-2<17:ASPAGC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMBINED SYSTEM; IDENTIFICATION; METABOLITES; PREPSTATION;
Keywords:
anabolic steroids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Munoz-Guerra, JA Higher Sports Council, Doping Control Lab, C Greco S-N, Madrid 28040, Spain Higher Sports Council C Greco S-N Madrid Spain 28040 ain
Citazione:
E. Haber et al., "Automated sample preparation and gas chromatographic-mass spectrometric analysis of urinary androgenic anabolic steroids", J CHROMAT B, 755(1-2), 2001, pp. 17-26

Abstract

This paper presents an automated method for extracting anabolic agents from urine samples for their GC-MS analysis by selected-ion monitoring. The sample preparation was carried out in a Hewlett-Packard 7686 SPE PrepStation system. Each 0.6-ml aliquot was hydrolyzed, extracted, dried and trimethylsilyl (TMS) derivatized in a 2-ml vial without any hands-on labor. When sample preparation was finished 2 mul of the extract was injected into the gas chromatograph by split (1:10) mode. Due to the small amount of free space in the 2-ml vials for handling the sample, parameters like time of hydrolysis, type of shaking, number of extractions and some TMS derivatization parameters had to be adjusted to achieve the best recovery for all of the compounds in the screening. Manual and automated sample preparation schemes were compared in terms of linearity, precision, accuracy, limit of detection andrecovery data. When large concentrations were analyzed using the automatedmethod no carry-over effect was observed. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 19:36:54