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Titolo:
Reduction of telomerase activity in human liver cancer cells by a histone deacetylase inhibitor
Autore:
Nakamura, M; Saito, H; Ebinuma, H; Wakabayashi, K; Saito, Y; Takagi, T; Nakamoto, N; Ishii, H;
Indirizzi:
Keio Univ, Dept Internal Med, Sch Med, Shinjuku Ku, Tokyo 1608582, Japan Keio Univ Tokyo Japan 1608582 Sch Med, Shinjuku Ku, Tokyo 1608582, Japan
Titolo Testata:
JOURNAL OF CELLULAR PHYSIOLOGY
fascicolo: 3, volume: 187, anno: 2001,
pagine: 392 - 401
SICI:
0021-9541(200106)187:3<392:ROTAIH>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEPATOCELLULAR-CARCINOMA; BUTYRATE-INDUCED APOPTOSIS; REVERSE-TRANSCRIPTASE HTERT; H-RAS PROTOONCOGENE; SODIUM-BUTYRATE; MESSENGER-RNA; HCC-T; CATALYTIC SUBUNIT; BCL-2 EXPRESSION; INTERFERON-ALPHA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Saito, H Keio Univ, Dept Internal Med, Sch Med, Shinjuku Ku, 35 Shinanomachi, Tokyo1608582, Japan Keio Univ 35 Shinanomachi Tokyo Japan 1608582 okyo1608582, Japan
Citazione:
M. Nakamura et al., "Reduction of telomerase activity in human liver cancer cells by a histone deacetylase inhibitor", J CELL PHYS, 187(3), 2001, pp. 392-401

Abstract

The presence of telomerase has been demonstrated recently in many different malignancies. Several reports documented that in human hepatocellular carcinoma, the level of telomerase activity parallels its differentiation stage. In the present study, the effect of the differentiation-inducing agent sodium butyrate on telomerase activity in four human liver cancer cell lineswas investigated using the telomeric repeat amplification protocol. We assayed telomerase activity before and after butyrate treatment and in cell cycle synchronized non-dividing quiescent cells. In addition, telomerase reverse transcriptase levels were measured at the mRNA level. All four cell lines possessed high but not identical levels of telomerase activity. Telomerase activity was significantly reduced by treatment with sodium butyrate as well as trichostatin A in a dose- and time-dependent fashion, paralleling the reduction of cell proliferation. Although methotrexate, hydroxyurea, andcolchicine synchronized the cell cycle at G1,S, and G2/M, respectively, and thereby also caused proliferating cells to cease dividing and become quiescent, in this case telomerase activity remained essentially unaltered compared to the control cultures. Moreover, levels of mRNA encoding telomerase reverse transcriptase were not always significantly altered by either sodium butyrate treatment or cell cycle synchronization. These results suggest that sodium butyrate, as a histone deacetylase inhibitor, effectively reduces telomerase activity without affecting transcription levels of the reversetranscriptase component. I. Cell. Physiol. 187: 392-401, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:17:12