Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Inhibition of JNK by overexpression of the JNK binding domain of JIP-1 prevents apoptosis in sympathetic neurons
Autore:
Harding, TC; Xue, LZ; Bienemann, A; Haywood, D; Dickens, M; Tolkovsky, AM; Uney, JB;
Indirizzi:
Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England Univ Cambridge Cambridge England CB2 1QW hem, Cambridge CB2 1QW, England Univ Bristol, Univ Res Ctr Neuroendocrinol, Bristol BS2 8HW, Avon, EnglandUniv Bristol Bristol Avon England BS2 8HW Bristol BS2 8HW, Avon, England Univ Bristol, MRC, Ctr Synapt Plast, Bristol BS2 8HW, Avon, England Univ Bristol Bristol Avon England BS2 8HW Bristol BS2 8HW, Avon, England Univ Leicester, Dept Med, Leicester LE1 7RH, Leics, England Univ Leicester Leicester Leics England LE1 7RH er LE1 7RH, Leics, England
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 7, volume: 276, anno: 2001,
pagine: 4531 - 4534
SICI:
0021-9258(20010216)276:7<4531:IOJBOO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; N-TERMINAL KINASE; C-JUN; PROTEIN-KINASE; NERVOUS-SYSTEM; EXPRESSION; ACTIVATION; STRESS; PHOSPHORYLATION; PATHWAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Tolkovsky, AM Univ Cambridge, Dept Biochem, Tennis Court Rd, Cambridge CB21QW, England Univ Cambridge Tennis Court Rd Cambridge England CB2 1QW nd
Citazione:
T.C. Harding et al., "Inhibition of JNK by overexpression of the JNK binding domain of JIP-1 prevents apoptosis in sympathetic neurons", J BIOL CHEM, 276(7), 2001, pp. 4531-4534

Abstract

Studies in non-neuronal cells show that c-Jun N-terminal kinases (JNK) play a key role in apoptotic cell death. In some neurons JNK is also thought to initiate cell death by the activation of c-Jun, JNK inhibition has been achieved pharmacologically by inhibiting upstream kinases, but there has been no direct demonstration that inhibition of JNK can prevent neuronal death. We have therefore examined whether the JNK binding domain (JBD) of JNK-interacting protein-1 (JIP-1, a scaffold protein and specific inhibitor of JNK) can inhibit c-Jun phosphorylation and support the survival of sympathetic neurons deprived of NGF. We show that expression of the JBD in >80% of neurons was sufficient to prevent the phosphorylation of c-Jun and its nuclear accumulation as well as abrogate neuronal cell death induced by NGF deprivation. JBD expression also preserved the capacity of mitochondria to reduce MTT. Interestingly, although the PTB domain of JIP was reported to interact with rhoGEF, expression of the JBD domain was sufficient to localize theprotein to the membrane cortex and growth cones. Hence, JNK activation is a key event in apoptotic death induced by NGF withdrawal, where its point of action lies upstream of mitochondrial dysfunction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 07:43:56