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Titolo:
Searching the point of no return in Helicobacter pylori life: necrosis and/or programmed death?
Autore:
Cellini, L; Robuffo, I; Maraldi, NM; Donelli, G;
Indirizzi:
Fac Med, Dept Biomed Sci, I-66100 Chieti, Italy Fac Med Chieti Italy I-66100 Med, Dept Biomed Sci, I-66100 Chieti, Italy Inst Normal Morphol, CNR, Inst Normal & Pathol Cytomorphol, Chieti, Italy Inst Normal Morphol Chieti Italy al & Pathol Cytomorphol, Chieti, Italy IOR, CNR, Inst Normal Pathol Cytomorphol, Bologna, Italy IOR Bologna Italy , CNR, Inst Normal Pathol Cytomorphol, Bologna, Italy Ist Super Sanita, Dept Ultrastruct, I-00161 Rome, Italy Ist Super Sanita Rome Italy I-00161 ept Ultrastruct, I-00161 Rome, Italy
Titolo Testata:
JOURNAL OF APPLIED MICROBIOLOGY
fascicolo: 5, volume: 90, anno: 2001,
pagine: 727 - 732
SICI:
1364-5072(200105)90:5<727:STPONR>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-DEATH; BACTERIAL-POPULATIONS; COCCOID FORMS; LOCALIZATION; INFECTION; SYSTEMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Cellini, L Fac Med, Dept Biomed Sci, Via Vestini 31, I-66100 Chieti, ItalyFac Med Via Vestini 31 Chieti Italy I-66100 6100 Chieti, Italy
Citazione:
L. Cellini et al., "Searching the point of no return in Helicobacter pylori life: necrosis and/or programmed death?", J APPL MICR, 90(5), 2001, pp. 727-732

Abstract

Aims: Ultrastructural and molecular studies to support the hypothesis of programmed cell death in Helicobacter pylori were conducted. Methods and Results: Evidence of programmed death in H. pylori is providedthrough electron microscopic detection and cytochemical labelling of electrondense bodies (EDB), containing packaged DNA in coccoid cells, resemblingmicronuclei of apoptotic eukaryotic cells. This morphological evidence is also supported by DNA cleavage in homogeneous fragments of about 100 base pairs. Programmed cell death was observed in H. pylori cultures at 37 degreesC, with a maximum of 37.5% of EDB coccoid cells after 7 days. The non-permissive temperature of 4 degreesC anticipated this process, with 40% of EDB coccoid forms within 3 days, and it remained substantially unaffected during the observation time of 14 days. Conclusion: In these experiments, deprivation of nutrients and a non-permissive temperature acted as a powerful trigger for programmed cell death. Significance and Impact of the Study: Helicobacter pylori bacterial populations, under stressing stimuli, can respond with programmed cell suicide asa means of species preservation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 15:37:53