Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Regulation of interleukin-10 secretion by histamine in TH2 cells and splenocytes
Autore:
Osna, N; Elliott, K; Khan, MM;
Indirizzi:
Creighton Univ, Dept Pharmaceut & Adm Sci, Omaha, NE 68178 USA Creighton Univ Omaha NE USA 68178 armaceut & Adm Sci, Omaha, NE 68178 USA
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 1, volume: 1, anno: 2001,
pagine: 85 - 96
SICI:
1567-5769(200101)1:1<85:ROISBH>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-A; MESSENGER-RNA EXPRESSION; HUMAN T-CELLS; NF-KAPPA-B; INTERFERON-GAMMA; TYROSINE PHOSPHORYLATION; SIGNAL-TRANSDUCTION; IL-12 PRODUCTION; GENE-EXPRESSION; MONOCYTES;
Keywords:
histamine; interleukin-10; protein kinase A; tyrphostin; TH2 cloned cells; splenocytes; interferon gamma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Khan, MM Creighton Univ, Dept Pharmaceut & Adm Sci, Omaha, NE 68178 USA Creighton Univ Omaha NE USA 68178 & Adm Sci, Omaha, NE 68178 USA
Citazione:
N. Osna et al., "Regulation of interleukin-10 secretion by histamine in TH2 cells and splenocytes", INT IMMUNO, 1(1), 2001, pp. 85-96

Abstract

Interleukin-10 is a potent suppressive factor that down-regulates cellularimmune response via inhibition of the production of Till cytokines. Histamine shifts: the TH1/TH2 balance from TH1 to TH2 cytokines making the effects of histamine on IL-10 secretion an important factor in this switch. This study was designed to assess the role of histamine in the regulation of IL-10 production and the involvement of PKA and STAT factors in this process. TH2 cells (D10.G4.1) and AKR/j splenocytes were pretreated with histamine at a concentration range of 10(-8)-10(-5) M for 1 h and then activated with PMA + ionomycin or anti-CD3 For 24 h. The supernatants were collected and tested for IL-10 content by ELISA. Histamine stimulated IL-10 production in TH2 cells in a dose-dependent manner that was reversed by both H1- and H2-receptor antagonists and by PKA inhibitors H8 and Rp-cAMPS. Tyrphostin also reversed the stimulation of IL-10 secretion by histamine, indicating that STAT factors were involved in this process, The up-regulation of IL-10 production by histamine in splenocytes was accompanied by inhibitory effects of histamine on IFN gamma production. The pretreatment of splenocytes with histamine in the presence of anti-IL-10 abrogated histamine-mediated inhibition of IFN gamma production suggesting that the effects of histamine on IFN gamma secretion were regulated by IL-10 in multi-cell system. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 09:10:18