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Titolo:
Chronic opioid treatment of the mouse thymoma cell lines R1.G1 and R1EGO leads to down-regulation of the kappa opioid receptor without desensitization of adenylyl cyclase activity
Autore:
Martin-Kleiner, I; Bidlack, JM;
Indirizzi:
Rudjer Boskovic Inst, Div Mol Med, Zagreb 10001, Croatia Rudjer Boskovic Inst Zagreb Croatia 10001 Mol Med, Zagreb 10001, Croatia Univ Rochester, Sch Med & Dent, Dept Physiol & Pharmacol, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 Pharmacol, Rochester, NY 14642 USA
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 1, volume: 1, anno: 2001,
pagine: 13 - 20
SICI:
1567-5769(200101)1:1<13:COTOTM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING-SITES; AGONIST U50,488H; IMMUNE-SYSTEM; MACROPHAGES; PEPTIDES; INHIBITION; PROTEIN; MU; LYMPHOCYTES; EXPRESSION;
Keywords:
kappa-opioids; mouse thymoma; adenylyl cyclase; receptor down-regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Martin-Kleiner, I Rudjer Boskovic Inst, Div Mol Med, Bijenicka C54, Zagreb10001, Croatia Rudjer Boskovic Inst Bijenicka C54 Zagreb Croatia 10001
Citazione:
I. Martin-Kleiner e J.M. Bidlack, "Chronic opioid treatment of the mouse thymoma cell lines R1.G1 and R1EGO leads to down-regulation of the kappa opioid receptor without desensitization of adenylyl cyclase activity", INT IMMUNO, 1(1), 2001, pp. 13-20

Abstract

Kappa opioid agonists alter some immune functions of macrophages, and T- and B-lymphocytes. The mouse thymoma cell lines R1.G1 and R1EGO express onlyK-opioid receptors and these kappa -opioid receptors are coupled to an inhibitory GTP-binding regulatory protein. Binding of kappa -opioid agonists to the opioid receptor leads to the inhibition of adenylyl cyclase activity in these cells. In this study, an acute (15 min) and chronic (24 h) treatment of R1.G1 and R1EGO cell with a potent K-opioid agonist (-)U50,488 (100 nM) was studied to determine if a kappa -opioid agonist altered receptor number and/or desensitization of adenylyl cyclase activity in these two cell lines. Chronic treatment of both R1.G1 and R1EGO cells with (-)U50, 488 leadto down-regulation of the kappa -opioid receptor, measured as a decrease of approximately 50% in the B-max value for the binding of [H-3]U69,593. Thebinding affinity(K-d value) was not affected after chronic treatment either in R1.G1 or R1EGO cells. There was no difference in the magnitude of inhibition of adenylyl cyclase activity by (-)U50,488 between the acute (15 min) and chronic (24-h) treatment in both cell lines R1.G1 and R1EGO. This study indicates that chronic opioid treatment of mouse thymoma R1.G1 and R1EGOcell lines leads to down-regulation of the receptor, without desensitization. This phenomenon was observed in R1.1 parent mouse thymoma cell line andrecently in CHO cells expressing K-opioid receptor. This study demonstrates that unlike some neuronal preparations, chronic opioid treatment of the thymoma cell lines resulted in receptor down-regulation without desensitization. (C) 2001 Elsevier Science B,V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 15:48:47