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Titolo:
Expression of CYP6B1 and CYP6B3 cytochrome P450 monooxygenases and furanocoumarin metabolism in different tissues of Papilio polyxenes (Lepidoptera :Papilionidae)
Autore:
Petersen, RA; Zangerl, AR; Berenbaum, MR; Schuler, MA;
Indirizzi:
Univ Illinois, Dept Entomol, Urbana, IL 61801 USA Univ Illinois Urbana ILUSA 61801 ois, Dept Entomol, Urbana, IL 61801 USA Univ Illinois, Dept Cell & Struct Biol, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 ell & Struct Biol, Urbana, IL 61801 USA
Titolo Testata:
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
fascicolo: 6-7, volume: 31, anno: 2001,
pagine: 679 - 690
SICI:
0965-1748(20010427)31:6-7<679:EOCACC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCIBLE CYTOCHROME-P450; FAT-BODY; BLACK SWALLOWTAIL; HOUSE-FLY; LINEAR FURANOCOUMARINS; PYRETHROID RESISTANCE; HELICOVERPA-ARMIGERA; HELIOTHIS-VIRESCENS; MOLECULAR-CLONING; GENE-EXPRESSION;
Keywords:
cytochrome P450 monooxygenases; furanocoumarin metabolism; mRNA expression; Papilio polyxenes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Petersen, RA Univ Illinois, Dept Entomol, 320 Morrill Hall,505 S Goodwin Ave, Urbana, IL 61801 USA Univ Illinois 320 Morrill Hall,505 S Goodwin Ave Urbana IL USA 61801
Citazione:
R.A. Petersen et al., "Expression of CYP6B1 and CYP6B3 cytochrome P450 monooxygenases and furanocoumarin metabolism in different tissues of Papilio polyxenes (Lepidoptera :Papilionidae)", INSEC BIO M, 31(6-7), 2001, pp. 679-690

Abstract

The CYP6B1 and CYP6B3 cytochrome P450 monooxygenases in the midgut of the black swallowtail participate in the metabolism of toxic furanocoumarins present in its host plants. In this study, biochemical analyses indicate thatthe fat body metabolizes significant amounts of the linear furanocoumarinsbergapten and xanthotoxin after larvae feed on xanthotoxin. Northern analyses of the combined CYP6B1/3 transcript expression patterns indicate that transcripts in this P450 subfamily are induced in the midgut and fat body byxanthotoxin. Semi-quantitative RT-PCR analyses of individual CYP6B1/CYP6B3mRNAs indicate that CYP6B1 transcripts are induced by xanthotoxin in all tissues examined and that CYP6B3 transcripts are induced in the fat body only. These results indicate that the fat body participates in the P450-mediated metabolism of excess furanocoumarins unmetabolized by the midgut. Although transcripts of both genes were detected and CYP6B1 transcripts were induced by xanthotoxin in the integument, furanocoumarin metabolism was not detected. Comparison of these P450 promoters with the promoters of alcohol dehydrogenase genes expressed in the fat bodies of several Drosophila species suggest that the xanthotoxin inducibilities of these P450 genes in fat bodies are regulated by elements other than those modulating expression of Adh genes. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 07:07:05