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Titolo:
Hepatic Kupffer cells: The portal that permits infection of hepatocytes bymalarial sporozoites?
Autore:
Barnwell, JW;
Indirizzi:
Ctr Dis Control & Prevent, Natl Ctr Infect Dis, Div Parasit Dis, Atlanta, GA 30341 USA Ctr Dis Control & Prevent Atlanta GA USA 30341 Dis, Atlanta, GA 30341 USA
Titolo Testata:
HEPATOLOGY
fascicolo: 5, volume: 33, anno: 2001,
pagine: 1331 - 1333
SICI:
0270-9139(200105)33:5<1331:HKCTPT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMODIUM-BERGHEI SPOROZOITES; HEPARAN-SULFATE PROTEOGLYCANS; INVASION IN-VITRO; CIRCUMSPOROZOITE PROTEIN; SURFACE; THROMBOSPONDIN; MACROPHAGES; FALCIPARUM; MEMBRANE; PARASITE;
Tipo documento:
Editorial Material
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Barnwell, JW Ctr Dis Control & Prevent, Natl Ctr Infect Dis, Div Parasit Dis, F-13,Bldg22B,4770 Buford Highway, Atlanta, GA 30341 USA Ctr Dis Control& Prevent F-13,Bldg 22B,4770 Buford Highway Atlanta GA USA 30341
Citazione:
J.W. Barnwell, "Hepatic Kupffer cells: The portal that permits infection of hepatocytes bymalarial sporozoites?", HEPATOLOGY, 33(5), 2001, pp. 1331-1333

Abstract

Malaria sporozoites ha ve to cross the layer of sinusoidal liver cells to reach their initial site of multiplication in the mammalian host, the hepatocytes. To determine the sinusoidal cell type sporozoites use for extravasation, endothelia or Kupffer cells, we quantified sporozoite adhesion to andinvasion of sinusoidal cells isolated from rat liver. In vitro invasion assays reveal that Plasmodium berghei and P. yoelii sporozoites attach to andenter Kupffer cells, but not sinusoidal endothelia. Unlike hepatocytes andother nonphagocytic cells, which are invaded in vitro only within the first hour of parasite exposure, the number of intracellular sporozoites in Kupffer cells increases for up to 12 hours. By confocal and electron microscopy, sporozoites are enclosed in a vacuole that does not colocalize with lysosomal markers. Inhibition of phagocytosis with gadolinium chloride has no effect on Kupffer cell invasion, but abolishes phagocytosis of inactivated sporozoites. Furthermore, sporozoites traverse in vitro from Kupffer cells to hepatocytes where they eventually develop into exoerythrocytic schizonts. Thus, malaria sporozoites selectively recognize and actively invade Kupffer cells, avoid phagosomal acidification, and safely passage through these phagocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 04:06:11