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Titolo:
Effects of estrous cycle and steroid replacement on the expression of leptin and uncoupling proteins in adipose tissue in the rat
Autore:
Luukkaa, V; Savontaus, E; Rouru, J; Virtanen, KA; Boss, O; Huhtaniemi, I; Koulu, M; Pesonen, U; Huupponen, R;
Indirizzi:
Univ Turku, Dept Pharmacol & Clin Pharmacol, FIN-20520 Turku, Finland UnivTurku Turku Finland FIN-20520 n Pharmacol, FIN-20520 Turku, Finland
Titolo Testata:
GYNECOLOGICAL ENDOCRINOLOGY
fascicolo: 2, volume: 15, anno: 2001,
pagine: 103 - 112
SICI:
0951-3590(200104)15:2<103:EOECAS>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESTRADIOL-TREATED RATS; CIRCULATING LEPTIN; LUTEINIZING-HORMONE; FEMALE RATS; BODY-COMPOSITION; MENSTRUAL-CYCLE; GENE-EXPRESSION; SERUM LEPTIN; OBESE GENE; SHORT-TERM;
Keywords:
leptin; uncoupling protein; UCP; gonadal hormones; estradiol; progesterone; estrous cycle; ovariectomy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Luukkaa, V Univ Turku, Dept Pharmacol & Clin Pharmacol, Kiinamyllynkatu 10, FIN-20520Turku, Finland Univ Turku Kiinamyllynkatu 10 Turku Finland FIN-20520 Finland
Citazione:
V. Luukkaa et al., "Effects of estrous cycle and steroid replacement on the expression of leptin and uncoupling proteins in adipose tissue in the rat", GYNECOL END, 15(2), 2001, pp. 103-112

Abstract

Among other actions, leptin has been suggested to increase energy expenditure and to modulate the menstrual cycle. In fact, the main effect of leptinseems to be modulating the sympathetic nervous system and gonadotropin-releasing hormone pulsatility. We investigated whether changes in the plasma steroid concentrations during the estrous cycle and after ovariectomy and steroid replacement can modulate plasma leptin levels, adipose tissue leptin mRNA expression, and some of the candidates for mediating energy expenditure (uncoupling proteins (UCP) 1, 2, and 3 mRNA) in white and brown adipose tissue. Rats in estrous cycle or ovariectomized vats with or without estradiol or progesterone replacement therapy for 18 days were studied. Plasma leptin, insulin, estradiol and progesterone were measured with radio-immunoassays. Leptin mRNA expression was measured in subcutaneous, periovarian and mesenteric white adipose tissue and in interscapular brown adipose tissue. Expression of UCP 1, 2, and 3 mRNA in periovarian white and brown adipose tissue was analyzed. Plasma leptin levels were significantly decreased in the estrous (1.1 +/- 0.4 ng/ml) compared with the pro-estrous (1.7 +/- 0.4 ng/ml, F = 3.0, p = 0.046) phase of cycle. UCP1 mRNA levels in brown adipose tissue were move elevated during pro-estrus than during metestrus (F = 3.17, p = 0.039). Gene expressions of leptin, UCP2 or UCP3 mRNA did not change significantly during the cycle. In ovariectomized rats, estradiol and/or progesterone treatment had no effect on plasma leptin levels. Gene expression analysis of leptin and UCP1, 2 and 3 in adipose tissue was not affected by steroid replacement. In conclusion, the estrous cycle appears to have a minor effect on modulation of leptin and uncoupling proteins. Only plasma leptin levels and expression of UCP1 mRNA are modestly elevated during the estrous cycle in the vat. Since estrogen and/or progesterone substitution in ovariectomized vats does not affect circulating leptin concentration or expression of leptin and UCPs in adipose tissue, it is unlikely that steroids play a major role in their regulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 22:23:44