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Titolo:
Development and assignment of bovine-specific PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probes
Autore:
Gautier, M; Laurent, P; Hayes, H; Eggen, A;
Indirizzi:
INRA, Dept Genet Anim, Lab Genet Biochim & Cytogenet, F-78352 Jouy En Josas, France INRA Jouy En Josas France F-78352 togenet, F-78352 Jouy En Josas, France
Titolo Testata:
GENETICS SELECTION EVOLUTION
fascicolo: 2, volume: 33, anno: 2001,
pagine: 191 - 200
SICI:
0999-193X(200103/04)33:2<191:DAAOBP>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; INTERNATIONAL-CONFERENCE; DOMESTIC-ANIMALS; CATTLE; STANDARDIZATION; KARYOTYPES; SYNTENY; MAP;
Keywords:
bovine; BAC library; cytogenetics; mapping; Texas standard;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Eggen, A INRA, Dept Genet Anim, Lab Genet Biochim & Cytogenet, F-78352 Jouy En Josas, France INRA Jouy En Josas France F-78352 F-78352 Jouy En Josas, France
Citazione:
M. Gautier et al., "Development and assignment of bovine-specific PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probes", GEN SEL EVO, 33(2), 2001, pp. 191-200

Abstract

In 1996, Popescu et al. published the Texas standard nomenclature of the bovine karyotype in which 31 marker genes, already mapped in man, were chosen to permit unambiguous identification and numbering of each bovine chromosome. However, specific PCR systems were not available for each marker gene thus preventing the assignment of part of these markers by somatic cell hybrid analysis. In addition, some difficulties remained with the nomenclatureof BTA25, BTA27 and BTA29. In this work, specific PCR systems were developed for each of the marker genes except VIL1 (see results), from either existing bovine or human sequences, and a bovine BAC library was screened to obtain the corresponding BAC clones. These PCR systems were used successfullyto confirm the assignment of each marker gene (except for LDHA, see results) by analysis on the INRA hamster-bovine somatic cell hybrid panel. The difficulties observed for LDHA and VIL1 are probably due to the fact that these genes belong to large gene families and therefore suggest that they may not be the most appropriate markers for a standardisation effort. This panel of BACs is available to the scientific community and has served as a basis for the establishment of a revised standard nomenclature of bovine chromosomes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 09:53:58