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Titolo:
Microcell-mediated transfer of chromosome 4 into HeLa cells suppresses telomerase activity
Autore:
Backsch, C; Wagenbach, N; Nonn, M; Leistritz, S; Stanbridge, E; Schneider, A; Durst, M;
Indirizzi:
FSU Jena, Frauenklin, Abt Frauenheilkunde, D-07743 Jena, Germany FSU JenaJena Germany D-07743 Abt Frauenheilkunde, D-07743 Jena, Germany FSU Jena, Inst Med Stat Informat & Dokumentat, Jena, Germany FSU Jena Jena Germany nst Med Stat Informat & Dokumentat, Jena, Germany Univ Calif Irvine, Coll Med, Dept Microbiol & Mol Genet, Irvine, CA 92717 USA Univ Calif Irvine Irvine CA USA 92717 l & Mol Genet, Irvine, CA 92717 USA
Titolo Testata:
GENES CHROMOSOMES & CANCER
fascicolo: 2, volume: 31, anno: 2001,
pagine: 196 - 198
SICI:
1045-2257(200106)31:2<196:MTOC4I>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PAPILLOMAVIRUS TYPE-16; CELLULAR SENESCENCE; GENETIC-ANALYSIS; INDEFINITE DIVISION; CERVICAL-CARCINOMA; HUMAN-FIBROBLASTS; DNA; IMMORTALIZATION; IDENTIFICATION; KERATINOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Durst, M FSU Jena, Frauenklin, Abt Frauenheilkunde, Bachstr 18, D-07743 Jena, Germany FSU Jena Bachstr 18 Jena Germany D-07743 , D-07743 Jena, Germany
Citazione:
C. Backsch et al., "Microcell-mediated transfer of chromosome 4 into HeLa cells suppresses telomerase activity", GENE CHROM, 31(2), 2001, pp. 196-198

Abstract

Telomerase activity can be detected in most human cancers and immortal cell lines. In contrast, the lack of telomerase activity in normal diploid fibroblasts has been correlated with progressive reduction of telomere lengthsto critically short sizes followed by the cessation of cell division and the onset of senescence. Several investigators have provided evidence for the localization of a telomerase suppressor gene on chromosome 3. The aim of our study was to determine whether other chromosomes are involved in telomerase repression. Beside human chromosome 3 (serving as positive control), chromosomes 4, 6, and 11 were introduced into HeLa cells via microcell-mediated chromosome transfer. Telomerase activity from different hybrid cell lysates was determined at an early time point after fusion using a Telomerase ELISA kit. Strong repression of telomerase activity was only found in a subset of HeLa hybrids in which chromosome 3 or chromosome 4 had been introduced. Telomerase suppression induced by chromosome 3 or 4 transfer was paralleled by a high frequency (30% or 43%, respectively) of a senescent-like phenotype. Chromosomes 6 and I I,the functional loss of which is also implicated in cervical cancer, had no effect. These results indicate that normal human chromosomes 3 and 4 carry a gene or genes that suppress telomerase activity and induce cellular senescence in HeLa cells. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 00:43:35