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Titolo:
Natural antisense transcripts are detected in different cell lines and tissues of cats infected with feline immunodeficiency virus
Autore:
Briquet, S; Richardson, J; Vanhee-Brossollet, C; Vaquero, C;
Indirizzi:
CHU Pitie Salpetriere, INSERM, U511, F-75013 Paris, France CHU Pitie Salpetriere Paris France F-75013 , U511, F-75013 Paris, France Inst Cochin Genet Mol, CNRS, UPR415, F-75014 Paris, France Inst Cochin Genet Mol Paris France F-75014 UPR415, F-75014 Paris, France GENSET SA, Intellectual Property Dept, F-75008 Paris, France GENSET SA Paris France F-75008 tual Property Dept, F-75008 Paris, France
Titolo Testata:
GENE
fascicolo: 2, volume: 267, anno: 2001,
pagine: 157 - 164
SICI:
0378-1119(20010418)267:2<157:NATADI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHEMOKINE RECEPTOR CXCR4; MOLECULAR-BIOLOGY; SHARED USAGE; RNA; REPLICATION; SEQUENCE; IDENTIFICATION; LENTIVIRUS; EXPRESSION; ELEMENTS;
Keywords:
endogenous; RNA; lentivirus; cat; AIDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Vaquero, C CHU Pitie Salpetriere, INSERM, U511, 91 Bd Hop, F-75013 Paris, France CHU Pitie Salpetriere 91 Bd Hop Paris France F-75013 s, France
Citazione:
S. Briquet et al., "Natural antisense transcripts are detected in different cell lines and tissues of cats infected with feline immunodeficiency virus", GENE, 267(2), 2001, pp. 157-164

Abstract

Feline immunodeficiency virus (FIV) is a lentivirus inducing an AIDS-like disease in cats, thus providing an interesting model for AIDS study. FIV and HIV-1 possess a similar genomic arrangement of structural and non-structural genes, whose expression is regulated by related genetic mechanisms. On the DNA strand complementary to the HIV-1 envelope messenger, an open reading frame (ORF) has been identified which encompasses the Rev Responsive Element (RRE), a cis-acting element critical to HIV gene expression. This ORF was highly conserved among HIV-1 isolates and had the potential to encode ahydrophobic protein. A corresponding antisense transcript was detected in cells infected with HIV-1, and appeared to encode an antisense protein. In the present study, we have identified a well-conserved antisense ORF, also coincident with the RRE region, in the genome of 21 FIV isolates. The predicted protein, 103 amino acids in length, is highly hydrophobic, as is the case for that of HIV-1. In addition, we have shown that a corresponding transcript, complementary to the transmembrane sequence of the Fl[V envelope gene, was produced in different cellular and viral contexts, that is, ex vivoand in vivo in FIV infected cell lines and tissues of infected cats, respectively. Expression of antisense transcripts might therefore be of general importance in the natural history of retroviruses. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:28:03