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Titolo:
Troglitazone stimulates basolateral rheogenic Na+/HCO3- cotransport activity in rabbit proximal straight tubules
Autore:
Muto, S; Miyata, Y; Imai, M; Asano, Y;
Indirizzi:
Jichi Med Sch, Dept Nephrol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Dept Pharmacol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan
Titolo Testata:
EXPERIMENTAL NEPHROLOGY
fascicolo: 3, volume: 9, anno: 2001,
pagine: 191 - 197
SICI:
1018-7782(2001)9:3<191:TSBRNC>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONVOLUTED TUBULE; MESANGIAL CELLS; RAT; SEGMENTS; MEMBRANE; NEPHRON; CS-045; EXIT; PH;
Keywords:
troglitazone; edema; proximal straight tubule; rheogenic Na+/HCO3- cotransport electrophysiology; intracellular pH measurement;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Muto, S Jichi Med Sch, Dept Nephrol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 3290498, Japan
Citazione:
S. Muto et al., "Troglitazone stimulates basolateral rheogenic Na+/HCO3- cotransport activity in rabbit proximal straight tubules", EXP NEPHROL, 9(3), 2001, pp. 191-197

Abstract

Thiazolidinedione derivatives, new insulin-sensitizing antidiabetic agents, are expected to have potential clinical use. Since These drugs cause edema in a variable proportion of patients, we examined whether troglitazone (Tro) has direct action on Na+ transport of rabbit proximal straight tubule perfused in vitro. For this purpose, we measured basolateral membrane voltage (V-B) by conventional microelectrode techniques and intracellular pH (pH(i)) by microscopic fluorescence spectrophotometry with a pH-sensitive fluorescent dye, 2 ' ,7 ' -bis-2-carboxyethyl-5-carboxyfluorescein. Tro at 50 muM in the bath significantly depolarized both transepithelial voltage and V-B. To examine whether the basolateral rheogenic Na+/HCO3- cotransport activity is affected by Tro, we observed V-B deflection upon abrupt 10-fold decrease in bath HCO; in the absence and presence of Tro. The apparent transference number of HCO3-(t(HCO3)), as calculated from the V-B deflection, was significantly greater in the presence of Tro (50 muM) than that seen in its absence. Tro caused cell acidification and increased the intracellular acidification rates (dpH(i)/dt) upon abrupt 10-fold decreases in bath HCO3- andNa+ concentrations. The stimulatory effects of Tro on t(HCO3) and dpH(i)/dt were dose dependent between 5 and 50 muM, but they were unaffected at 0.5muM. From these results, we conclude that Tro acts on the proximal straight tubule and stimulates the basolateral rheogenic Na+/HCO3- cotransport activity. The stimulatory action of Tro may partly account for edema formation. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 02:34:06