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Titolo:
Monoclonal antibody against Porphyromonas gingivalis hemagglutinin inhibits hemolytic activity
Autore:
Hosogi, Y; Hayakawa, M; Abiko, Y;
Indirizzi:
Nihon Univ, Sch Dent, Dept Biochem, Chiba 2718587, Japan Nihon Univ Chiba Japan 2718587 Dent, Dept Biochem, Chiba 2718587, Japan
Titolo Testata:
EUROPEAN JOURNAL OF ORAL SCIENCES
fascicolo: 2, volume: 109, anno: 2001,
pagine: 109 - 113
SICI:
0909-8836(200104)109:2<109:MAAPGH>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIPLE GENE-PRODUCTS; CYSTEINE PROTEINASE; BACTEROIDES-GINGIVALIS; PASSIVE-IMMUNIZATION; PERIODONTAL-DISEASE; VIRULENCE FACTORS; COLONIZATION; ERYTHROCYTES; CONSTRUCTION; ADHERENCE;
Keywords:
Porphyromonas gingivalis; hemolysis; hemagglutinin; monoclonal antibody; passive immunization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Abiko, Y Nihon Univ, Sch Dent, Dept Biochem, 2-870-1 Sakaecho Nishi, Chiba2718587,Japan Nihon Univ 2-870-1 Sakaecho Nishi Chiba Japan 2718587 8587,Japan
Citazione:
Y. Hosogi et al., "Monoclonal antibody against Porphyromonas gingivalis hemagglutinin inhibits hemolytic activity", EUR J OR SC, 109(2), 2001, pp. 109-113

Abstract

Porphyromonas gingivalis has been implicated as an important pathogen in the development of adult periodontitis. This bacterium possesses hemagglutinating and hemolytic activities to attach and lyse erythrocytes. Hemolysis by this oral pathogen functions to provide heme-containing molecules for growth in the periodontal pocket. We previously constructed a monoclonal antibody using P. gingivalis vesicles as the immunogen, designated as MAb-Pg-vc,which inhibited vesicle-associated hemagglutinating activity. Furthermore,we cloned the gene encoding 130-kDa hemagglutinin (130-kDa HAG) and identified its functional motif for attachment to erythrocytes. Generally, bacterial cell attachment to erythrocytes is an important initial step for expressing hemolysis activity. In the present study, we examined the effect of MAb-Pg-vc on the hemolytic activity of P. gingivalis cells. The MAb-Pg-vc significantly inhibited the hemolytic activity and, further, this inhibitory activity was reduced by the synthetic peptide of the 130-kDa HAG functional motif.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 05:34:35