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Titolo:
Morphine-induced stimulation of pituitary-adrenocortical activity is mediated by activation of nitric oxide in the early stages of postnatal life in the rat
Autore:
Lesage, J; Bernet, F; Montel, V; Dupouy, JP;
Indirizzi:
Univ Lille 1, Lab Neuroendocrinol Dev, UPRES EA 2701, F-59655 Villeneuve Dascq, France Univ Lille 1 Villeneuve Dascq France F-59655 55 Villeneuve Dascq, France
Titolo Testata:
EUROPEAN JOURNAL OF ENDOCRINOLOGY
fascicolo: 4, volume: 144, anno: 2001,
pagine: 441 - 451
SICI:
0804-4643(200104)144:4<441:MSOPAI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICOTROPIN-RELEASING FACTOR; ADRENAL AXIS; ENDOGENOUS OPIOIDS; PRENATAL MORPHINE; NEWBORN RAT; CRF RELEASE; RECEPTORS; OPIATE; BRAIN; HORMONE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Lesage, J Univ Lille 1, Lab Neuroendocrinol Dev, UPRES EA 2701, Bat SN4, F-59655 Villeneuve Dascq, France Univ Lille 1 Bat SN4 Villeneuve Dascq France F-59655 cq, France
Citazione:
J. Lesage et al., "Morphine-induced stimulation of pituitary-adrenocortical activity is mediated by activation of nitric oxide in the early stages of postnatal life in the rat", EUR J ENDOC, 144(4), 2001, pp. 441-451

Abstract

Objective: The first aim of the present study was to determine if morphine, a prototypic mu -opioid agonist drug, affects pituitary-adrenocortical activity in developing rat pups (first and second weeks of postnatal life). The second aim of this study was to explore, in vivo, if nitric oxide (NO) could be involved in the neurohormonal response to morphine in the early stages of postnatal life. Methods: Plasma ACTH and corticosterone concentrations were determined by RIA in rat pups (n = 5-14 rats/experimental group) after they had been killed by decapitation. In a first experiment, 1-day and 1- and 2-week-old ratswere treated s.c. with morphine (20 mg/kg) or with vehicle (0.9% NaCl) andkilled 5-90 min later. In a second experiment, 2-week-old pups were pretreated s.c. with naltrexone (NAL: 0.4 mg/kg or 10 mg/kg), and injected 1 h later with either morphine (20 mg/kg) or vehicle, and killed 30 min later. Some pups injected with only NAL were killed 60 or 90 min later. On the otherhand, pups injected with NAL (10 mg/kg) or NAL and morphine were killed 30min later. In a third experiment, 2-week-old pups were pretreated s.c, with N-omega -nitro arginine methylester (L-NAME: 30 mg/kg or 100 mg/kg), and injected 1 h later with either morphine (20 mg/kg) or vehicle, and killed 30 min later. Moreover, some pups injected with L-NAME (100 mg/kg) or L-NAMEwith morphine were killed 30 min later. In a final experiment, pups were injected s.c. with either S-nitroso-N-acetylpenicillamine (SNAP: 5 mg/kg) orvehicle, and killed 60 or 90 min later,Results: Morphine administered to rat pups elicited marked rises in both ACTH and corticosterone secretion. Moreover, these responses increased with advancing postnatal age. In 2-week-old rat pups, NAL, a competitive antagonist at mu -opioid receptors, administered alone increased both plasma ACTH and corticosterone concentrations 30 min later, L-NAME, a specific NO synthase inhibitor, did not affect plasma ACTH and corticosterone concentrations30 min later when administered alone. NAL, when concomitant-ly administered with morphine, was unable to block morphine responses. In contrast, morphine responses were blocked by pretreatment (60 min before) with NAL or withL-NAME, Acute injection of SNAP increased both ACTH and corticosterone release. Conclusion: Our results suggest that opioids have controversial effects onpituitary-adrenocortical activity in the early postnatal period in the rat, and that endogenous NO is one of the major factors in the response of thepituitary-adrenocortical axis to morphine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 09:20:20