Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Embryonic retinoic acid synthesis is essential for heart morphogenesis in the mouse
Autore:
Niederreither, K; Vermot, J; Messaddeq, N; Schuhbaur, B; Chambon, P; Dolle, P;
Indirizzi:
Coll France, Inst Genet & Biol Mol & Cellulaire, ULP, CNRS,INSERM, F-67404Illkirch, France Coll France Illkirch France F-67404 CNRS,INSERM, F-67404Illkirch, France
Titolo Testata:
DEVELOPMENT
fascicolo: 7, volume: 128, anno: 2001,
pagine: 1019 - 1031
SICI:
0950-1991(200104)128:7<1019:ERASIE>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEVELOPING MAMMALIAN HEART; NEURAL CREST CELLS; RXR-ALPHA; MUTANT MICE; POSTIMPLANTATION DEVELOPMENT; CARDIAC MORPHOGENESIS; DEHYDROGENASE TYPE-2; TRANSCRIPTION FACTOR; BINDING-PROTEINS; QUAIL EMBRYO;
Keywords:
cardiomyocyte differentiation; heart development; left-right patterning; mouse mutant; myocardium; Raldh2; retinaldehyde dehydrogenase; retinoids; Tbx5;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Dolle, P Coll France, Inst Genet & Biol Mol & Cellulaire, ULP, CNRS,INSERM, BP 163,CU Strasbourg, F-67404 Illkirch, France Coll France BP 163,CU Strasbourg Illkirch France F-67404 France
Citazione:
K. Niederreither et al., "Embryonic retinoic acid synthesis is essential for heart morphogenesis in the mouse", DEVELOPMENT, 128(7), 2001, pp. 1019-1031

Abstract

Retinoic acid (RA), the active derivative of vitamin A, has been implicated in various steps of cardiovascular development, but its contribution to early heart morphogenesis has not been clearly established in a mammalian system, To block endogenous RA synthesis, we have disrupted the gene encodingRALDH2, the first retinaldehyde dehydrogenase whose expression has been detected during early mouse post-implantation development. We describe here the heart abnormalities of the RA-deficient Raldh2 mutants that die in uteroat gestational day 10.5. The embryonic heart tube forms properly, but fails to undergo rightward looping and, instead, forms a medial distended cavity, Expression of early heart determination factors is not altered in mutants, and the defect in heart looping does not appear to involve the Nodal/Lefty/Pitx2 pathway. Histological and molecular analysis reveal distinct anteroposterior components in the mutant heart tube, although posterior chamber (atria and sinus venosus) development is severely impaired. Instead of forming trabeculae, the developing ventricular myocardium consists of a thick layer of loosely attached cells. Ultrastructural analysis shows that most ofthe ventricular wall consists of prematurely differentiated cardiomyocytes, whereas undifferentiated cells remain clustered rostrally. We conclude that embryonic RA synthesis is required for realization of heart looping, development of posterior chambers and proper differentiation of ventricular cardiomyocytes, Nevertheless, the precise location of this synthesis may not be crucial, as these defects can mostly be rescued by systemic (maternal) RA administration. However, cardiac neural crest cells cannot be properly rescued in Raldh2(-/-) embryos, leading to outflow tract septation defects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 22:13:46