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Titolo:
Validation of a chiral capillary electrochromatographic method for metoprolol on a teicoplanin stationary phase
Autore:
Carlsson, E; Wikstrom, H; Owens, PK;
Indirizzi:
AstraZeneca R&D Molndal, Analyt Dev, S-43183 Molndal, Sweden AstraZeneca R&D Molndal Molndal Sweden S-43183 , S-43183 Molndal, Sweden
Titolo Testata:
CHROMATOGRAPHIA
fascicolo: 7-8, volume: 53, anno: 2001,
pagine: 419 - 424
SICI:
0009-5893(200104)53:7-8<419:VOACCE>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
REVERSED-PHASE; PHARMACEUTICAL ANALYSIS; ENANTIOMER SEPARATION; ELECTROPHORESIS; DESIGNS; SILICA; DRUGS; CEC;
Keywords:
capillary electrochromatography; chiral separation; experimental design; metoprolol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Owens, PK AstraZeneca R&D Molndal, Analyt Dev, S-43183 Molndal, Sweden AstraZeneca R&D Molndal Molndal Sweden S-43183 Molndal, Sweden
Citazione:
E. Carlsson et al., "Validation of a chiral capillary electrochromatographic method for metoprolol on a teicoplanin stationary phase", CHROMATOGR, 53(7-8), 2001, pp. 419-424

Abstract

A chiral capillary electrochromatographic (CEC) method for determination of the enantiomeric purity of ei ther enantiomer of metoprolol has been validated, High resolution a nd efficiency separations (R-S = 2.5 and 80 000 plates m(-1), respectively) were achieved by use of a teicoplanin chiral stationary phase in the polar organic mode. Method validation showed that detection linearity, robustness, accuracy, and repeatability were adequate. The method was also shown to be sufficiently sensitive for the determination ofa mi nor enantiomer; the limit of quantitation (LOQ) was determined to be 0.09% of the peak area of the enantiomer under investigation. A similar commercial column was subsequently evaluated by use of the validated method and found to yield results for metoprolol comparable with those obtained on the home-packed columns. Acceptable separations on this commercial column were also obtained for other beta -blocking drugs; those for alprenolol were particularly noteworthy (R-s = 3.8 and 265 000 plates m(-1)).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:26:52