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Titolo:
Amino acids characterization by reversed-phase liquid chromatography. Partial least-squares modeling of their transport properties
Autore:
Silva, MF; Chipre, LF; Raba, J; Luco, JM;
Indirizzi:
Univ Nacl San Luis, Fac Quim Bioquim & Farm, Area Quim Analit, RA-5700 SanLuis, Argentina Univ Nacl San Luis San Luis Argentina RA-5700 RA-5700 SanLuis, Argentina Univ Nacl San Luis, Fac Quim Bioquim & Farm, Lab Alimentos, RA-5700 San Luis, Argentina Univ Nacl San Luis San Luis Argentina RA-5700 A-5700 San Luis, Argentina
Titolo Testata:
CHROMATOGRAPHIA
fascicolo: 7-8, volume: 53, anno: 2001,
pagine: 392 - 400
SICI:
0009-5893(200104)53:7-8<392:AACBRL>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-BRAIN-BARRIER; DISTRIBUTION COEFFICIENTS; CLERODANE DITERPENOIDS; CHEMICAL DESCRIPTORS; ANTIFEEDANT ACTIVITY; GLOBULAR-PROTEINS; HYDROPHOBICITY; LIPOPHILICITY; RESIDUES; DESIGN;
Keywords:
column liquid chromatography; alpha-amino acids; partial least-squares analysis; orthogonal signal correction; QSRR and QSAR analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Silva, MF Univ Nacl San Luis, Fac Quim Bioquim & Farm, Area Quim Analit, RA-5700 SanLuis, Argentina Univ Nacl San Luis San Luis Argentina RA-5700 nLuis, Argentina
Citazione:
M.F. Silva et al., "Amino acids characterization by reversed-phase liquid chromatography. Partial least-squares modeling of their transport properties", CHROMATOGR, 53(7-8), 2001, pp. 392-400

Abstract

In the present work, the 20 coded free amino acids (AAs) were characterized with respect to their lipophilic behavior by means of reversed-phase liquid chromatography (RPLC) and using two octadecyl silica (ODS) stationary phases and phosphate-buffered mobile phases at three pH values. To complementthe results obtained with the ODS phases, one phenyl stationary phase was also used. The retention behavior of the AAs under study was investigated by means of the multiple linear regression (MLR) technique and several molecular descriptors were used as predictor variables. The results show that the obtained chromatographic parameters provide a different type of information from both, the currently used lipophilic scales and several chromatographic hydrophobicity scales based upon derivatives of the AAs. The relationships between the obtained retention data and both the brain uptake data, reported data on kinetic parameters, and lipid bilayer affinities for several AAs, multivariate projection techniques such as partial least-squares (PLS) regression were used. The results indicate that the combination of RPLC techniques and chemometrics is a promising approach in assessing the hydrophobic amino acid-membrane interactions.

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Documento generato il 29/03/20 alle ore 17:17:15