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Titolo:
Pseudomonas aeruginosa ExoT inhibits in vitro lung epithelial wound repair
Autore:
Geiser, TK; Kazmierczak, BI; Garrity-Ryan, LK; Matthay, MA; Engel, JN;
Indirizzi:
Univ Calif San Francisco, Cardiovasc Res Inst, San Francisco, CA 94143 USAUniv Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA Univ CalifSan Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA
Titolo Testata:
CELLULAR MICROBIOLOGY
fascicolo: 4, volume: 3, anno: 2001,
pagine: 223 - 236
SICI:
1462-5814(200104)3:4<223:PAEIIV>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GTPASE-ACTIVATING PROTEIN; EXOENZYME-S; AIRWAY EPITHELIUM; CELL INJURY; RHO-GTPASES; IN-VITRO; CYTOTOXICITY; VIRULENCE; POLARITY; INVASION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Engel, JN Univ Calif San Francisco, Cardiovasc Res Inst, San Francisco, CA94143 USA Univ Calif San Francisco San Francisco CA USA 94143 A 94143 USA
Citazione:
T.K. Geiser et al., "Pseudomonas aeruginosa ExoT inhibits in vitro lung epithelial wound repair", CELL MICROB, 3(4), 2001, pp. 223-236

Abstract

The nosocomial pathogen Pseudomonas aeruginosa causes clinical infection in the setting of pre-existing epithelial tissue damage, an association thatis mirrored by the increased ability of P. aeruginosa to bind, invade and damage injured epithelial cells in vitro. In this study, we report that P. aeroginosa inhibits the process of epithelial wound repair in vitro throughthe type III-secreted bacterial protein ExoT, a GTPase-activating protein (GAP) for Rho family GTPases. This inhibition primarily targets cells at the edge of the wound, and causes actin cytoskeleton collapse, cell rounding and cell detachment. ExoT-dependent inhibition of wound repair is mediated through the GAP activity of this bacterial protein, as mutations in ExoT that alter the conserved arginine (R149) within the GAP domain abolish the ability of P. aeruginosa to inhibit wound closure. Because ExoT can also inhibit P. aeruginosa internalization by phagocytes and epithelial cells, this protein may contribute to the in vivo virulence of P. aeruginosa by allowing organisms both to overcome local host defences, such as an intact epithelial barrier, and to evade phagocytosis by immune effector cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:41:11