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Titolo:
EspA filament-mediated protein translocation into red blood cells
Autore:
Shaw, RK; Daniell, S; Ebel, F; Frankel, G; Knutton, S;
Indirizzi:
Univ Birmingham, Inst Child Hlth, Birmingham B4 6NH, W Midlands, England Univ Birmingham Birmingham W Midlands England B4 6NH W Midlands, England Univ London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AZ,England Univ London Imperial Coll Sci Technol & Med London England SW7 2AZ gland Inst Pasteur, Unite Genet Mol, F-75724 Paris, France Inst Pasteur Paris France F-75724 Unite Genet Mol, F-75724 Paris, France
Titolo Testata:
CELLULAR MICROBIOLOGY
fascicolo: 4, volume: 3, anno: 2001,
pagine: 213 - 222
SICI:
1462-5814(200104)3:4<213:EFPTIR>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTEROPATHOGENIC ESCHERICHIA-COLI; EPITHELIAL-CELLS; HOST-CELLS; SUPRAMOLECULAR STRUCTURE; PATHOGENICITY ISLAND; SECRETED PROTEIN; PLASMA-MEMBRANE; ADHERENCE; SURFACE; SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Knutton, S Univ Birmingham, Inst Child Hlth, Birmingham B4 6NH, W Midlands, England Univ Birmingham Birmingham W Midlands England B4 6NH , England
Citazione:
R.K. Shaw et al., "EspA filament-mediated protein translocation into red blood cells", CELL MICROB, 3(4), 2001, pp. 213-222

Abstract

Type III secretion allows bacteria to inject effector proteins into host cells. In enteropathogenic Escherichia coli (EPEC), three type III secreted proteins, EspA, EspB end EspD, have been shown to be required for translocation of the Tir effector protein into host cells. EspB and EspD have been proposed to form a pore in the host cell membrane, whereas EspA, which formsa large filamentous structure bridging bacterial and host cell surfaces, is thought to provide a conduit for translocation of effector proteins between pores in the bacterial and host cell membranes. Type III secretion has been correlated with an ability to cause contact-dependent haemolysis of redblood cells (RBCs) in vitro. As EspA filaments link bacteria and the host cell, we predicted that intimate bacteria-RBC contact would not be requiredfor EPEC-induced haemolysis and, therefore, in this study we investigated the interaction of EPEC with monolayers of RBCs attached to polylysine-coated cell culture dishes, EPEC caused total RBC haemolysis in the absence of centrifugation and osmoprotection studies were consistent with the insertion of a hydrophilic pore into the RBC membrane. Cell attachment and haemolysis involved interaction between EspA filaments and the RBC membrane and wasdependent upon a functional type III secretion system and on EspD, whereasEPEC lacking EspB still caused some haemolysis, Following haemolysis, onlyEspD was consistently detected in the RBC membrane. This study shows that intimate bacteria-RBC membrane contact is not a requirement for EPEC-induced haemolysis; it also provides further evidence that EspA filaments are a conduit for protein translocation and that EspD may be the major component of a translocation pore in the host cell membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:40:58