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Titolo:
Dendritic cells are early cellular targets of Listeria monocytogenes afterintestinal delivery and are involved in bacterial spread in the host
Autore:
Pron, B; Boumaila, C; Jaubert, F; Berche, P; Milon, G; Geissmann, F; Gaillard, JL;
Indirizzi:
Hop Raymond Poincare, Microbiol Lab, F-92380 Garches, France Hop Raymond Poincare Garches France F-92380 Lab, F-92380 Garches, France INSERM, U411, F-75015 Paris, France INSERM Paris France F-75015INSERM, U411, F-75015 Paris, France Fac Med Necker Enfants Malad, Pathol Lab, F-75015 Paris, France Fac Med Necker Enfants Malad Paris France F-75015 F-75015 Paris, France Inst Pasteur, Unite Immunophysiol & Parasitisme Intracellulaire, F-75015 Paris, France Inst Pasteur Paris France F-75015 Intracellulaire, F-75015 Paris, France Hop Necker Enfants Malad, CNRS, UMR 8603, F-75015 Paris, France Hop NeckerEnfants Malad Paris France F-75015 603, F-75015 Paris, France
Titolo Testata:
CELLULAR MICROBIOLOGY
fascicolo: 5, volume: 3, anno: 2001,
pagine: 331 - 340
SICI:
1462-5814(200105)3:5<331:DCAECT>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; COLONY-STIMULATING FACTOR; ACTIN-BASED MOVEMENT; LANGERHANS CELLS; INTRACELLULAR GROWTH; EPIDEMIC LISTERIOSIS; MAMMALIAN-CELLS; SURFACE PROTEIN; LYMPH-NODES; T-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Gaillard, JL Hop Raymond Poincare, Microbiol Lab, 104 Blvd R Poincare, F-92380 Garches,France Hop Raymond Poincare 104 Blvd R Poincare Garches France F-92380
Citazione:
B. Pron et al., "Dendritic cells are early cellular targets of Listeria monocytogenes afterintestinal delivery and are involved in bacterial spread in the host", CELL MICROB, 3(5), 2001, pp. 331-340

Abstract

We studied the sequence of cellular events leading to the dissemination ofListeria monocytogenes from the gut to draining mesenteric lymph nodes (MLNs) by confocal microscopy of immunostained tissue sections from a rat ligated ileal loop system. OX-62- positive cells beneath the epithelial lining of Peyer's patches (PPs) were the first Listeria targets identified after intestinal inoculation. These cells had other features typical of dendritic cells (DCs): they were large, pleiomorphic and major histocompatibility complex class IIhi. Listeria were detected by microscopy in draining MLNs as early as 6 h after inoculation. Some 80-90% of bacteria were located in the deep paracortical regions, and 100% of the bacteria were present in OX-62-positive cells. Most infected cells contained more than five bacteria each, suggesting that they had arrived already loaded with bacteria. At later stages, the bacteria in these areas were mostly present in EDI-positive mononuclear phagocytes. These cells were also infected by an actA mutant defective in cell-to-cell spreading. This suggests that Listeria are transported byDCs from PPs to the deep paracortical regions of draining MLNs and are then transmitted to other cell populations by mechanisms independent of ActA. Another pathway of dissemination to MLNs was identified, probably involvingfree Listeria and leading to the infection of ED3-positive mononuclear phagocytes in the subcapsular sinus and adjacent paracortical areas. This study provides evidence that DCs are major cellular targets of L. monocytogenesin PPs and that DCs may be involved in the early dissemination of this pathogen. DCs were not sites of active bacterial replication, making these cells ideal vectors of infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 12:21:26