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Titolo:
Frequent c-Ha-ras gene mutations in rat mammary carcinomas induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine
Autore:
Hokaiwado, N; Asamoto, M; Cho, YM; Imaida, K; Shirai, T;
Indirizzi:
Nagoya City Univ, Sch Med, Dept Pathol 1, Mizuho Ku, Nagoya, Aichi 4678601, Japan Nagoya City Univ Nagoya Aichi Japan 4678601 Nagoya, Aichi 4678601, Japan
Titolo Testata:
CANCER LETTERS
fascicolo: 2, volume: 163, anno: 2001,
pagine: 187 - 190
SICI:
0304-3835(20010226)163:2<187:FCGMIR>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HETEROCYCLIC AMINES; COOKED FOODS; GLAND TUMORS; CARCINOGENESIS; COLON; INDUCTION; PHIP;
Keywords:
2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine; mammary tumor; H-ras; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Asamoto, M Nagoya City Univ, Sch Med, Dept Pathol 1, Mizuho Ku, Mizuho Cho, Nagoya, Aichi 4678601, Japan Nagoya City Univ Mizuho Cho Nagoya Aichi Japan 4678601 1, Japan
Citazione:
N. Hokaiwado et al., "Frequent c-Ha-ras gene mutations in rat mammary carcinomas induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine", CANCER LETT, 163(2), 2001, pp. 187-190

Abstract

2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), the most abundant heterocyclic amine in cooked meat and fish, is carcinogenic to the mammary glands of rats. Mutations in the H-ras gene were here examined in PhIP-induced mammary tumors of female F344 rats by the polymerase chain reaction followed by single strand conformation polymorphism analysis (PCR-SSCP) and restriction enzyme length polymorphism analysis (RFLP). Mutations in codon 12of the H-ras gene were detected in four out of 13 tumors by PCR-SSCP. Three of them were GGA to GAA, and one was GGA to GTA. However, by RFLP analysis, four additional mutations in codon 13 were also detected in the same samples. Two had a GGC to CGC mutation, and the other shifts were GGC to GAC and GGC to GTC. Therefore, overall eight out of 13 cases had H-ras gene mutations. These results indicate that changes in H-ras function may play important roles in PhIP-induced mammary carcinogenesis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 10:13:00