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Titolo:
Relative quantitation of p53 and MDM2 gene expression in leiomyosarcoma; real-time semi-quantitative reverse transcription-polymerase chain reaction
Autore:
Miyajima, K; Tamiya, S; Oda, Y; Adachi, T; Konomoto, T; Toyoshiba, H; Masuda, K; Tsuneyoshi, M;
Indirizzi:
Kyushu Univ, Grad Sch Med Sci, Dept Anat Pathol, Dept Pathol 2,Higashi Ku,Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ol 2,Higashi Ku,Fukuoka 8128582, Japan Kyushu Univ, Grad Sch Med Sci, Dept Med Informat Sci, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ci, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ, Grad Sch Med Sci, Dept Clin Radiol, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ol, Higashi Ku, Fukuoka 8128582, Japan
Titolo Testata:
CANCER LETTERS
fascicolo: 2, volume: 164, anno: 2001,
pagine: 177 - 188
SICI:
0304-3835(20010326)164:2<177:RQOPAM>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOFT-TISSUE SARCOMAS; MALIGNANT FIBROUS HISTIOCYTOMA; MINIMAL RESIDUAL DISEASE; CELLULAR TUMOR-ANTIGEN; MESSENGER-RNA; ABNORMALITIES; MUTATION; PROTEIN; AMPLIFICATION; PCR;
Keywords:
leiomyosarcoma; p53; MDM2; real-time semi-quantitative reverse transcription-polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Tsuneyoshi, M Kyushu Univ, Grad Sch Med Sci, Dept Anat Pathol, Dept Pathol2,Higashi Ku,Maidashi 3-1-1, Fukuoka 8128582, Japan Kyushu Univ Maidashi 3-1-1 Fukuoka Japan 8128582 582, Japan
Citazione:
K. Miyajima et al., "Relative quantitation of p53 and MDM2 gene expression in leiomyosarcoma; real-time semi-quantitative reverse transcription-polymerase chain reaction", CANCER LETT, 164(2), 2001, pp. 177-188

Abstract

To investigate the etiology of leiomyosarcoma, we examined abnormalities of p53 and its regulation in 13 cases of leiomyosarcoma using fresh tumor specimens. We estimated p53 and MDM2 mRNA level and MDM2 gene amplification using a real-time semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) based on the TaqMan fluorescence method. We also used immunohistochemistry (IHC) for p53 and MDM2 protein overexpression, polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and direct sequencing to detect p53 mutation. Eight of the 13 cases (62%) showed an overexpression of p53 protein on IHC and eight of 13 cases (62%) had p53 gene point mutations. Five of the 13 cases (38%) showed positive staining forMDM2 protein and only one case (7.7%) demonstrated MDM2 gene amplification. The relative p53 mRNA level of the tumors compared with normal tissue ranged from 1.14 to 12.19 arbitrary units (AU), and the MDM2 mRNA level rangedfrom 1.06 to 17.17 AU. The mRNA level in the p53-positive cases was higherthan in the negative cases (positive: 7.70 AU on average; negative: 3.38 AU on average; P = 0.0344). However, there was no significant correlation between the MDM2 mRNA level and other factors, such as p53 IHC, p53 mutation status, p53 mRNA level and MDM2 IHC. Our results indicate that p53 abnormalities are major events and that an increasing level of p53 mRNA is associated with an overexpression of p53 protein in leiomyosarcoma and they may play an important role in the tumorigenesis in this tumor. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 15:16:44