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Titolo:
Chemical force microscopy with active enzymes
Autore:
Fiorini, M; McKendry, R; Cooper, MA; Rayment, T; Abell, C;
Indirizzi:
Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England Univ Cambridge Cambridge England CB2 1EW hem, Cambridge CB2 1EW, England
Titolo Testata:
BIOPHYSICAL JOURNAL
fascicolo: 5, volume: 80, anno: 2001,
pagine: 2471 - 2476
SICI:
0006-3495(200105)80:5<2471:CFMWAE>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADHESION; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Rayment, T Univ Cambridge, Dept Chem, Lensfield Rd, Cambridge CB2 1EW, England Univ Cambridge Lensfield Rd Cambridge England CB2 1EW England
Citazione:
M. Fiorini et al., "Chemical force microscopy with active enzymes", BIOPHYS J, 80(5), 2001, pp. 2471-2476

Abstract

The adhesion forces have been measured between an atomic force microscope tip derivatized with an active enzyme, shikimate kinase, and an ATP mimic immobilized on a gold surface. Experiments with competitive binding of otherligands in solution show that the observed adhesion forces arise predominantly from specific interactions between the immobilized enzyme and surface-bound adenine derivative. These experiments represent a step in the development of a screening methodology based upon chemical force microscopy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:21:45