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Titolo:
Effect of fibroblast donor cell age and cell cycle on development of bovine nuclear transfer embryos in vitro
Autore:
Kasinathan, P; Knott, JG; Moreira, PN; Burnside, AS; Jerry, DJ; Robl, JM;
Indirizzi:
Univ Massachusetts, Dept Vet & Anim Sci, Amherst, MA 01003 USA Univ Massachusetts Amherst MA USA 01003 & Anim Sci, Amherst, MA 01003 USA
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 5, volume: 64, anno: 2001,
pagine: 1487 - 1493
SICI:
0006-3363(200105)64:5<1487:EOFDCA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSPLANT RABBIT EMBRYOS; REPLICATIVE LIFE-SPAN; SOMATIC-CELLS; PARTHENOGENETIC DEVELOPMENT; FETAL FIBROBLASTS; MAMMALIAN-CELLS; MATURED OOCYTES; IN-VITRO; ADULT; STAGE;
Keywords:
developmental biology; implantation/early development;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Robl, JM Univ Massachusetts, Dept Vet & Anim Sci, Amherst, MA 01003 USA Univ Massachusetts Amherst MA USA 01003 i, Amherst, MA 01003 USA
Citazione:
P. Kasinathan et al., "Effect of fibroblast donor cell age and cell cycle on development of bovine nuclear transfer embryos in vitro", BIOL REPROD, 64(5), 2001, pp. 1487-1493

Abstract

The effects of cell cycle stage and the age of the cell donor animal on invitro development of bovine nuclear transfer embryos were investigated. Cultures of primary bovine fibroblasts were established from animals of various ages, and the in vitro life span of these cell lines was analyzed. Fibroblasts from both fetuses and calves had similar in vitro life spans of approximately 30 population doublings (PDs) compared with 20 PDs in fibroblastsobtained from adult animals. When fibroblasts from both fetuses and adult animals were cultured as a population, the percentage of cells in G1 increased linearly with time, whereas the percentage of S-phase cells decreased proportionately. Furthermore, the percentage of cells in G1 at a given time was higher in adult fibroblasts than in fetal fibroblasts. To study the individual cells from a population, a shake-off method was developed to isolate cells in G1 stage of the cell cycle and evaluate the cell cycle characteristics of both fetal and adult fibroblasts from either 25% or 100% confluent cultures. Irrespective of the age, the mean cell cycle length in isolatedcells was shorter (9.6-15.5 h) than that observed for cells cultured as a population. Likewise, the length of the G1 stage in these isolated cells, as indicated by 5-bromo-deoxyuridine labeling, lasted only about 2-3 h. There were no differences in either the number of cells in blastocysts or the percentage of blastocysts between the embryos reconstructed with G1 cells from 25% or 100% confluent cultures of fetal or adult cell lines. This study suggests that there are substantial differences in cell cycle characteristics in cells derived from animals of different ages or cultured at differentlevels of confluence. However, these factors had no effect on in vitro development of nuclear transfer embryos.

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Documento generato il 31/10/20 alle ore 21:11:07