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Titolo:
Quantitative two-color fluorescence cross-correlation spectroscopy in the analysis of polymerase chain reaction
Autore:
Foldes-Papp, Z; Rigler, R;
Indirizzi:
Karolinska Inst, MBB, Dept Med Biophys, S-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden S-17177 phys, S-17177 Stockholm, Sweden
Titolo Testata:
BIOLOGICAL CHEMISTRY
fascicolo: 3, volume: 382, anno: 2001,
pagine: 473 - 478
SICI:
1431-6730(200103)382:3<473:QTFCSI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Keywords:
absolute molecule number; ligand binding; signal amplification; specific detection mode; theory of two-color fluorescence cross-correlation spectroscopy; two ligands with different colors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Rigler, R Karolinska Inst, MBB, Dept Med Biophys, S-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden S-17177 177 Stockholm, Sweden
Citazione:
Z. Foldes-Papp e R. Rigler, "Quantitative two-color fluorescence cross-correlation spectroscopy in the analysis of polymerase chain reaction", BIOL CHEM, 382(3), 2001, pp. 473-478

Abstract

We present results of an approach in which low-density labeled DNA itself provides an amplification of the cross-correlated fluorescent signal in thetwo-color cross- correlation function. Tetramethylrhodamine-4-dUTP and Cy5-dCTP are incorporated by polymerase chain reaction at multiple positions of the same 217 bp target DNA. We call this novel approach the 'two-color FCS signal amplification'. The signal amplification is an example for interactions of two ligands with different colors at multiple positions of the same target.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:39:25