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Titolo:
Isolation of plasma kallikrein by high efficiency affinity chromatography and its characterization
Autore:
Tada, M; Tsuda, Y; Wanaka, K; Hijikata-Okunomiya, A; Horie, N; Okamoto, U; Okamoto, S; Okada, Y;
Indirizzi:
Kobe Gakuin Univ, Fac Pharmaceut Sci, Nishi Ku, Kobe, Hyogo 6512180, JapanKobe Gakuin Univ Kobe Hyogo Japan 6512180 Ku, Kobe, Hyogo 6512180, Japan Kobe Gakuin Univ, High Technol Res Ctr, Nishi Ku, Kobe, Hyogo 6512180, Japan Kobe Gakuin Univ Kobe Hyogo Japan 6512180 Ku, Kobe, Hyogo 6512180, Japan Kobe Res Projects Thrombosis & Haemostasis, Tarumi Ku, Kobe, Hyogo 6550033, Japan Kobe Res Projects Thrombosis & Haemostasis Kobe Hyogo Japan 6550033 Japan Kobe Univ, Sch Med, Fac Hlth Sci, Kobe, Hyogo 6540142, Japan Kobe Univ Kobe Hyogo Japan 6540142 c Hlth Sci, Kobe, Hyogo 6540142, Japan Mukogawa Womens Univ, Sch Human Environm Sci, Nishinomiya, Hyogo 6338558, Japan Mukogawa Womens Univ Nishinomiya Hyogo Japan 6338558 Hyogo 6338558, Japan
Titolo Testata:
BIOLOGICAL & PHARMACEUTICAL BULLETIN
fascicolo: 5, volume: 24, anno: 2001,
pagine: 520 - 524
SICI:
0918-6158(200105)24:5<520:IOPKBH>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PURIFICATION METHOD; INHIBITORY ACTIVITY; HAGEMAN-FACTOR; KININ SYSTEM; ACTIVATION; PREKALLIKREIN; COAGULATION;
Keywords:
plasma kallikrein; affinity chromatography; plasma kallikrein selective inhibitor; PKSI-527; reverse phase-HPCL profile; molecular weight;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Okada, Y Kobe Gakuin Univ, Fac Pharmaceut Sci, Nishi Ku, Kobe, Hyogo 6512180, Japan Kobe Gakuin Univ Kobe Hyogo Japan 6512180 , Hyogo 6512180, Japan
Citazione:
M. Tada et al., "Isolation of plasma kallikrein by high efficiency affinity chromatography and its characterization", BIOL PHAR B, 24(5), 2001, pp. 520-524

Abstract

We established a simple method for the purification of human plasma kallikrein (PK) by affinity chromatography and characterized it by analytical reverse phase-HPLC and Time of Flight Mass Spectroscopy (TOF-RIS). The affinity resin (PKSI-Toyopearl) was synthesized using a selective synthetic inhibitor of plasma kallikrein (PKSI-527) as a ligand, The resin was found to have the highest efficiency in PK purification when the coupling ratio of PKSI-527 per resin was 9-14 mu mol/g. PK was purified 466-fold with a field of 83% from acetone-activated human plasma by affinity chromatography, The purity of PK thus obtained,vas confirmed by reverse phase-HPLC with a linear gradient of acetonitrile. The molecular weight of the purified PK was determined to be 86,151 by TOF-MS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 11:57:37