Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cyclic-AMP inhibits nitric oxide-induced apoptosis in human osteoblast: The regulation of caspase-3,-6,-9 and the release of cytochrome c in nitric oxide-induced apoptosis by cAMP
Autore:
Chae, HJ; Chae, SW; An, NH; Kim, JH; Kim, CW; Yoo, SK; Kim, HH; Lee, ZH; Kim, HR;
Indirizzi:
Wonkwang Univ, Sch Dent, Dept Dent Pharmacol, Iksan 570749, Chonbuk, SouthKorea Wonkwang Univ Iksan Chonbuk South Korea 570749 70749, Chonbuk, SouthKorea Wonkwang Univ, Sch Dent, Wonkwang Dent Res Inst, Iksan 570749, Chonbuk, South Korea Wonkwang Univ Iksan Chonbuk South Korea 570749 0749, Chonbuk, South Korea Chonbuk Natl Univ, Sch Med, Dept Pharmacol, Kwangju 501759, South Korea Chonbuk Natl Univ Kwangju South Korea 501759 Kwangju 501759, South Korea Chonbuk Natl Univ, Sch Med, Inst Cardiovasc Res, Kwangju 501759, South Korea Chonbuk Natl Univ Kwangju South Korea 501759 Kwangju 501759, South Korea Wonkwang Univ, Coll Pharm, Natl Res Lab Bone Metab, Kwangju 501759, South Korea Wonkwang Univ Kwangju South Korea 501759 ab, Kwangju 501759, South Korea Wonkwang Univ, Dept Gynecol, Natl Res Lab Bone Metab, Kwangju 501759, South Korea Wonkwang Univ Kwangju South Korea 501759 ab, Kwangju 501759, South Korea Wonkwang Univ, Sch Oriental Med, Natl Res Lab Bone Metab, Kwangju 501759, South Korea Wonkwang Univ Kwangju South Korea 501759 ab, Kwangju 501759, South Korea Chosun Univ, Res Ctr Proteinous Mat, Kwangju 501759, South Korea Chosun Univ Kwangju South Korea 501759 Mat, Kwangju 501759, South Korea
Titolo Testata:
BIOLOGICAL & PHARMACEUTICAL BULLETIN
fascicolo: 5, volume: 24, anno: 2001,
pagine: 453 - 460
SICI:
0918-6158(200105)24:5<453:CINOAI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEATH GENE CED-3; CELL-DEATH; INTERFERON-GAMMA; HL-60 CELLS; L-ARGININE; ENZYME; ACTIVATION; MODULATION; PROTEASE; NECROSIS;
Keywords:
NO; cAMP; apoptosis; osteoblast; caspase; cytochrome c;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, HR Wonkwang Univ, Sch Dent, Dept Dent Pharmacol, Iksan 570749, Chonbuk, SouthKorea Wonkwang Univ Iksan Chonbuk South Korea 570749 honbuk, SouthKorea
Citazione:
H.J. Chae et al., "Cyclic-AMP inhibits nitric oxide-induced apoptosis in human osteoblast: The regulation of caspase-3,-6,-9 and the release of cytochrome c in nitric oxide-induced apoptosis by cAMP", BIOL PHAR B, 24(5), 2001, pp. 453-460

Abstract

Nitric oxide (NO) induces apoptotic cell death and cAMP has a significantly protective effect on NO-induced cytotoxicity in human osteoblasts, MG-63 cells. Treatment with S-nitroso-N-acetylpenicillamine (SNAP) (0.6 mM) resulted in genomic DNA fragmentation, characteristic of apoptosis, However, concomitant incubation of the cells with tither DBcAMP or forskolin markedly inhibited SNAP-induced apoptosis in a dose-dependent manner. Furthermore, pretreatment of MG-63 cells with H-89 or KT5720, which is known to inhibit cAMP-dependent protein kinase (PKA), abolished the protective effect of DBcAMP and forskolin on SNAP-induced apoptosis, In this study, we explored the involvement of caspases in the regulatory mechanism of SNAP-induced apoptosis by cAMP. Our data show that DBcAMP or forskolin blocked SNAP-induced caspase-3-like cysteine protease activation and that H-89, a PKA inhibitor, reversed the cAMP-induced regulatory effect of caspase-3 like protease, Consistent with the results, cAMP inhibited the proteolytic cleavage of caspase-3, -6, -9 and cytochrome c release to cytoplasm. The inhibition of caspase-3activation did not block SNAP-induced cytochrome c release to cytoplasm. suggesting that caspase-3 activation may occur downstream of cytochrome c release, In summary, these findings show that the exposure of MG-63 cells to cAMP analogs renders them more resistant to NO-induced damage and suggests the presence of regulatory mechanisms of the cell death pathway by cAMP in which caspase-3, -6, and -9 and cytochrome c release scn es to mediate NO-induced apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 23:02:03