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Titolo:
Cloning and characterization of a human orphan family C G-protein coupled receptor GPRC5D
Autore:
Brauner-Osborne, H; Jensen, AA; Sheppard, PO; Brodin, B; Krogsgaard-Larsen, P; OHara, P;
Indirizzi:
Royal Danish Sch Pharm, Dept Med Chem, NeuroSci PharmaBiotec Res Ctr, DK-2100 Copenhagen, Denmark Royal Danish Sch Pharm Copenhagen Denmark DK-210000 Copenhagen, Denmark Zymogenet Inc, Seattle, WA 98105 USA Zymogenet Inc Seattle WA USA 98105Zymogenet Inc, Seattle, WA 98105 USA Royal Danish Sch Pharm, Dept Pharmaceut, Ctr Drug Design & Transport, DK-2100 Copenhagen, Denmark Royal Danish Sch Pharm Copenhagen Denmark DK-210000 Copenhagen, Denmark
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
fascicolo: 3, volume: 1518, anno: 2001,
pagine: 237 - 248
SICI:
0167-4781(20010416)1518:3<237:CACOAH>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-SENSING RECEPTOR; METABOTROPIC GLUTAMATE RECEPTORS; AMINO-TERMINAL DOMAIN; EXTRACELLULAR DOMAIN; SEQUENCE ALIGNMENT; MOLECULAR-CLONING; AGONIST-BINDING; LIGAND-BINDING; SITES; DIFFERENTIATION;
Keywords:
orphan G protein coupled receptor; GPRC5D; retinoic acid; gene induction; chimeric receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Brauner-Osborne, H Royal Danish Sch Pharm, Dept Med Chem, NeuroSci PharmaBiotec Res Ctr, 2 Univ Pk, DK-2100 Copenhagen, Denmark Royal Danish Sch Pharm 2 Univ Pk Copenhagen Denmark DK-2100
Citazione:
H. Brauner-Osborne et al., "Cloning and characterization of a human orphan family C G-protein coupled receptor GPRC5D", BBA-GENE ST, 1518(3), 2001, pp. 237-248

Abstract

Recently three orphan G-protein coupled receptors. RAIG1, GPRC5B and GPRC5C, with homology to members of family C (metabotropic glutamate receptor-like) have been identified. Using the protein sequences of these receptors asqueries we identified overlapping expressed sequence tags which were predicted to encode an additional subtype, The full length coding regions of mouse mGprc5d and human GPRC5D were cloned and shown to contain predicted openreading frames of 300 and 345 amino acids, respectively. GPRC5D has seven putative transmembrane segments and is expressed in the cell membrane. The four human receptor subtypes, which we assign to group 5 of family C GPCRs,show 31-42% amino acid sequence identity to each other and 20-25% sequenceidentity to the transmembrane domains of metabotropic glutamate receptor subtypes 2 and 3 and other family C members. In contrast to the remaining Family C members. the group 5 receptors have short amino terminal domains of some 30-50 amino acids. GPRC5D was shown to be clustered with RAIG1 on chromosome 12p13.3 and like RAIG1 and GPRC5B to consist of three exons, the first exon being the largest containing all seven transmembrane segments. GPRC5D mRNA is widely expressed in the peripheral system but all four receptorsshow distinct expression patterns. Interestingly, mRNA levels of all four group 5 receptors were found in medium to high levels in the kidney, pancreas and prostate and in low to medium levels in the colon and the small intestine. whereas other organs only express a subset of the genes. In an attempt to delineate the signal transduction pathway(s) of the orphan receptors:a series of chimeric receptors containing the amino terminal domain of thecalcium sensing receptor or metabotropic glutamate receptor subtype 1, andthe seven transmembrane domain of the orphan receptors were constructed and tested in binding and functional assays. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 22:37:26