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Titolo:
A MEK inhibitor, PD98059 enhances IL-1-induced NF-kappa B activation by the enhanced and sustained degradation of I kappa B alpha
Autore:
Funakoshi, M; Tago, K; Sonoda, Y; Tominaga, S; Kasahara, T;
Indirizzi:
Kyoritsu Coll Pharm, Dept Biochem & Immunol, Minato Ku, Tokyo 1058512, Japan Kyoritsu Coll Pharm Tokyo Japan 1058512 Minato Ku, Tokyo 1058512, Japan Jichi Med Sch, Dept Biochem, Minami Kawachi, Tochigi 3290433, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290433 Tochigi 3290433, Japan
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 1, volume: 283, anno: 2001,
pagine: 248 - 254
SICI:
0006-291X(20010427)283:1<248:AMIPEI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZINC FINGER PROTEIN; INTERLEUKIN-1 RECEPTOR; TNF-ALPHA; INDUCTION; PHOSPHORYLATION; PROTEASOME; COMPLEX; UBIQUITINATION; EXPRESSION; PROMOTER;
Keywords:
interleukin-1; NF-kappa B; PD98059; MEK; ERK; I kappa B alpha; proteasome;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Kasahara, T Kyoritsu Coll Pharm, Dept Biochem & Immunol, Minato Ku, 1-5-30Shibakoen, Tokyo 1058512, Japan Kyoritsu Coll Pharm 1-5-30 Shibakoen Tokyo Japan 1058512 apan
Citazione:
M. Funakoshi et al., "A MEK inhibitor, PD98059 enhances IL-1-induced NF-kappa B activation by the enhanced and sustained degradation of I kappa B alpha", BIOC BIOP R, 283(1), 2001, pp. 248-254

Abstract

Interleukin-1 (IL-1) mediates numerous host responses through rapid activation of nuclear factor-kappaB (NF-kappaB), but signal pathways leading to the NF-kappaB activation appear to be complicated and multiplex. We propose a novel regulatory system for NF-kappaB activation by the extracellular signal-related kinase (ERK) pathway. In a human glioblastoma cell line, T98G, IL-1-induced NF-kappaB activation was significantly augmented by the pretreatment of a specific MEK inhibitor, PD98059. In contrast, ectopic expression of a constitutive activated form of Raf (v-Raf) reduced IL-1-induced NF-kappaB activation, and this inhibition was completely reversed by PD98059. Interestingly, PD98059 sustained IL-1-induced NF-kappaB DNA binding activityby an eletrophoretic mobility shift assay and also I kappaB alpha degradation presumably by augmenting and sustaining the proteasome activation. Concomitantly, two NF-kappaB dependent genes, A20 and I kappaB alpha expressionwere prolonged with PD98059. These data suggested that MEK-ERK pathway exerts a regulatory effect on NF-kappaB activation, providing a novel insight on the role of MEK-ERK pathway. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 14:51:22