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Titolo:
Peptides encoded by exon 6 of VEGF inhibit endothelial cell biological responses and angiogenesis induced by VEGF
Autore:
Jia, HY; Jezequel, S; Lohr, M; Shaikh, S; Davis, D; Soker, S; Selwood, D; Zachary, I;
Indirizzi:
Univ Coll London, Rayne Inst, Dept Med, London WC1E 6JJ, England Univ CollLondon London England WC1E 6JJ t Med, London WC1E 6JJ, England Univ Coll London, Rayne Inst, Ark Therapeut Ltd, London WC1E 6JJ, England Univ Coll London London England WC1E 6JJ t Ltd, London WC1E 6JJ, England Univ Coll London, Wolfson Inst Biomed Res, London, England Univ Coll London London England olfson Inst Biomed Res, London, England Harvard Univ, Sch Med, Childrens Hosp, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 ed, Childrens Hosp, Boston, MA 02115 USA
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 1, volume: 283, anno: 2001,
pagine: 164 - 173
SICI:
0006-291X(20010427)283:1<164:PEBE6O>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR VEGF; TUMOR-GROWTH; SIGNAL-TRANSDUCTION; TYROSINE KINASES; 7-ENCODED DOMAIN; ORF VIRUS; RECEPTOR; NEUROPILIN-1; BINDING; PROTEIN;
Keywords:
KDR; neuropilin-1; apoptosis; migration; ERK; prostacyclin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Jia, HY Univ Coll London, Rayne Inst, Dept Med, 5 Univ St, London WC1E 6JJ, England Univ Coll London 5 Univ St London England WC1E 6JJ E 6JJ, England
Citazione:
H.Y. Jia et al., "Peptides encoded by exon 6 of VEGF inhibit endothelial cell biological responses and angiogenesis induced by VEGF", BIOC BIOP R, 283(1), 2001, pp. 164-173

Abstract

VEGF induces pathological angiogenesis and is an important target for the development of novel antiangiogenic molecules. In this study, we tested synthetic peptides based on the sequence of VEGF(189) for their ability to inhibit VEGF receptor binding and biological responses. Ne identified 12-aminoacid peptides derived from exon 6 that inhibited VEGF binding to HUVECs, VEGF-stimulated ERK activation, and prostacyclin production. These peptides inhibited VEGF-induced mitogenesis, migration, and VEGF-dependent survival of endothelial cells, but caused no increase in apoptosis in the absence ofVEGF. Exon 6-encoded peptides also caused a marked inhibition of VEGF-induced angiogenesis in vitro. Studies of effects of peptides on cross-linking of VEGF to its receptors and on binding of VEGF to porcine aortic endothelial cells expressing either KDR or neuropilin-1 showed that exon 6-encoded peptides effectively blocked the interaction of VEGF with both receptors. Exon 6-derived peptides caused release of bFGF from endothelial cells but inhibited bFGF-dependent ERK activation, cell proliferation and angiogenesis. Our findings indicate that VEGF exon 6-encoded peptides inhibit VEGF-induced angiogenesis, at least in part through inhibition of VEGF binding to KDR. In addition, exon 6-encoded peptides are also effective inhibitors of bFGF-mediated angiogenesis. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:19:39