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Titolo:
Morphological analysis of endocytosis in efferent ductules of estrogen receptor-alpha knockout male mouse
Autore:
Nakai, M; Bouma, J; Nie, R; Zhou, Q; Carnes, K; Lubahn, DB; Hess, RA;
Indirizzi:
Univ Illinois, Dept Vet Biosci, Urbana, IL 61801 USA Univ Illinois UrbanaIL USA 61801 , Dept Vet Biosci, Urbana, IL 61801 USA Univ Missouri, Dept Biochem, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 Dept Biochem, Columbia, MO 65211 USA Univ Missouri, Dept Child Hlth, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 pt Child Hlth, Columbia, MO 65211 USA
Titolo Testata:
ANATOMICAL RECORD
fascicolo: 1, volume: 263, anno: 2001,
pagine: 10 - 18
SICI:
0003-276X(20010501)263:1<10:MAOEIE>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPITHELIAL-CELLS; REPRODUCTIVE-TRACT; EXCURRENT DUCTS; MALE-MICE; FLUID REABSORPTION; NONCILIATED CELLS; MARMOSET MONKEY; RAT TESTIS; DIFFERENTIATION; EPIDIDYMIS;
Keywords:
alpha ERKO; efferent ductules; endocytosis; estrogen; mouse; reabsorption;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Hess, RA Univ Illinois, Dept Vet Biosci, 2001 S Lincoln Ave, Urbana, IL 61801 USA Univ Illinois 2001 S Lincoln Ave Urbana IL USA 61801 IL 61801 USA
Citazione:
M. Nakai et al., "Morphological analysis of endocytosis in efferent ductules of estrogen receptor-alpha knockout male mouse", ANAT REC, 263(1), 2001, pp. 10-18

Abstract

Lack of estrogen receptor (ER) results in fluid accumulation and dilation of the efferent ductules, suggesting that the role of estrogen and ER in the male reproductive tract is related to fluid reabsorption in the ductules. In the present study, endocytosis of the nonciliated cells of the efferentductules was compared morphologically between wild type (WT) and estrogen receptor-alpha, knockout (alpha ERKO) male mice. The epithelial cells lining the WT efferent ductules were tall columnar in shape, whereas those of the alpha ERKO were low columnar. Immunocytochemically, the nonciliated cellsof both genotypes showed positive reactions of sulfated glycoprotein-2, but the reaction products were reduced in amount in the alpha ERKC. Electron microscopy revealed that the nonciliated cells of the WT had numerous organelles for endocytosis such as coated pits and vesicles, tubules, endosomes,multivesicular bodies and lysosomes in the apical cytoplasm. These organelles were less developed in the nonciliated cells of the alpha ERKO. Morphometric analysis indicated that there was a significant reduction in area of endocytotic apparatus in the nonciliated cells of the aERKO compared with that of the WT. A tracer study using gold particles demonstrated that the nonciliated cells of both WT and aERKO efferent ductules were capable of taking up luminal contents. These results suggest that reabsorption of the luminal contents via endocytosis takes place in the efferent ductules but is greatly reduced in amount in the absence of ER alpha. Anat Rec 263:10-18, 2001. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 02/04/20 alle ore 00:11:35