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Titolo:
Microsatellite instability in transforming growth factor-beta 1 type II receptor gene in alveolar lining epithelial cells of idiopathic pulmonary fibrosis
Autore:
Mori, M; Kida, H; Morishita, H; Goya, S; Matsuoka, H; Arai, T; Osaki, T; Tachibana, I; Yamamoto, S; Sakatani, M; Ito, M; Ogura, T; Hayashi, S;
Indirizzi:
Osaka Univ, Grad Sch Med C4, Dept Mol Med, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 Mol Med, Suita, Osaka 5650871, Japan Natl Kinkichuo Hosp Chest Dis, Dept Internal Med, Sakai, Osaka, Japan NatlKinkichuo Hosp Chest Dis Sakai Osaka Japan Med, Sakai, Osaka, Japan Natl Toneyama Hosp, Dept Internal Med, Toyonaka, Osaka, Japan Natl Toneyama Hosp Toyonaka Osaka Japan rnal Med, Toyonaka, Osaka, Japan
Titolo Testata:
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
fascicolo: 4, volume: 24, anno: 2001,
pagine: 398 - 404
SICI:
1044-1549(200104)24:4<398:MIITGF>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
TGF-BETA RECEPTOR; INTERSTITIAL PNEUMONIA; COLORECTAL-CANCER; MUTATION ANALYSIS; EXPRESSION; TGF-BETA-1; PANCREAS; CLONING; COMPLEX; LESIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Mori, M Osaka Univ, Grad Sch Med C4, Dept Mol Med, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 5650871, Japan
Citazione:
M. Mori et al., "Microsatellite instability in transforming growth factor-beta 1 type II receptor gene in alveolar lining epithelial cells of idiopathic pulmonary fibrosis", AM J RESP C, 24(4), 2001, pp. 398-404

Abstract

It has been reported that transforming growth factor (TC F)-beta, which plays an integral role in the pathogenesis of idiopathic pulmonary fibrosis (IPF), suppresses proliferation of alveolar epithelial cells in vitro. Although hyperplastic lesions of alveolar lining epithelial cells (ALECs) are characteristic pathologic features of IPF, the mechanism of their involvementin the pathogenesis has not yet been extensively studied. On the assumption that the hyperplastic ALECs have escaped from the growth-inhibitory effects of TCF-beta, we searched for mutations in the microsatellite of the TCF-beta receptor type II (T beta RII) gene. To detect a deletion in the polyadenine tract in exon 3 of the T beta RII gene, cells were isolated by microdissection from lung sections of IPF patients, and DNA was extracted from these cells and amplified by high-fidelity polymerase chain reaction. A totalof 121 sites of hyperplastic ALECs from 11 IPF patients were analyzed, anda one-base-pair deletion was detected in nine sites from five patients. The mutation was also detected in smooth muscle-like cells of the thickened pulmonary artery. In some tissue areas where the deletion was detected, low T beta RII expression was confirmed by immunohistochemical staining. These data suggest that microsatellite instability in the T beta RII gene occurred in some lesions of hyperplastic ALECs in IPF, although at a low incidence, and that this genetic disorder might play a partial role in the pathologic changes of IPF.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 22:51:12