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Titolo:
Antibody testing against canine coronavirus by immunoperoxidase plaque staining
Autore:
Soma, T; Hara, M; Ishii, H; Yamamoto, S;
Indirizzi:
Marupi Lifetech Co Ltd, Vet Diagnost Lab, Ikeda, Osaka 5630011, Japan Marupi Lifetech Co Ltd Ikeda Osaka Japan 5630011 da, Osaka 5630011, Japan Azabu Univ, Sch Vet Med, Dept Microbiol, Sagamihara, Kanagawa, Japan AzabuUniv Sagamihara Kanagawa Japan robiol, Sagamihara, Kanagawa, Japan Sukagawa Anim Hosp, Sukagawa, Fukushima, Japan Sukagawa Anim Hosp Sukagawa Fukushima Japan , Sukagawa, Fukushima, Japan Azabu Univ, Coll Environm & Hlth Sci, Dept Immunol, Sagamihara, Kanagawa, Japan Azabu Univ Sagamihara Kanagawa Japan mmunol, Sagamihara, Kanagawa, Japan
Titolo Testata:
VETERINARY RESEARCH COMMUNICATIONS
fascicolo: 4, volume: 25, anno: 2001,
pagine: 327 - 336
SICI:
0165-7380(2001)25:4<327:ATACCB>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; DOGS; PARVOVIRUS; RESPONSES; INFECTION; PROTEINS; VIRUS; CATS;
Keywords:
antibody; canine coronavirus; diagnosis; diarrhoea; dog; immunoperoxidase plaque staining; neutralizing test; serology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Soma, T Marupi Lifetech Co Ltd, Vet Diagnost Lab, 103 Fushiocho, Ikeda, Osaka 5630011, Japan Marupi Lifetech Co Ltd 103 Fushiocho Ikeda Osaka Japan 5630011 pan
Citazione:
T. Soma et al., "Antibody testing against canine coronavirus by immunoperoxidase plaque staining", VET RES COM, 25(4), 2001, pp. 327-336

Abstract

The application of the immunoperoxidase (IP) plaque staining procedure (IPtest) to the diagnosis of canine coronavirus (CCV) infection was investigated. The IP test did not react with sera from either 15 specific pathogen-free (SPF) dogs or 7 SPF dogs immunized with a multivalent vaccine, including canine parvovirus type 2, canine distemper virus, canine adenovirus type 2, and canine parainfluenza virus. To compare the IP test with the neutralizing test (NT), sera from 240 healthy dogs and from 3 experimentally CCV-infected dogs were examined. All 60 sera positive for NT antibody were positive for IP antibody, and all 180 sera negative for NT antibody were negativefor IP antibody in the healthy dogs. The IP titres showed similar changes with time after CCV inoculation to those of the NT titres in the experimentally infected dogs. These findings indicate that the IP test specifically detected anti-CCV antibodies. When the IP test and NT were compared in dogs with diarrhoeic signs. 2.1% of 48 sera and 20.3% of 74 sera, which were allnegative for NT antibody, were positive for IP antibody in the dogs of under one year of age and at least one year of age, respectively. The difference between the IP and NT titres (log(10) [reciprocal of IP titre] - log(10)[reciprocal of NT titre]) for the diarrhoeic dogs of under one year of age(2.350 +/-0.931) was significantly larger than that for the healthy dogs (0.982 +/-0.447) (p <0.0001), the NT titre being negative or very low, despite a high IP titre in many diarrhoeic dogs. Hence, the IP test is more ableto detect anti-CCV antibodies, especially in dogs showing clinical signs. The IP-positivity rate was significantly higher in the diarrhoeic dogs of under one year of age (48.7%) than in the healthy dogs (25.0%) (chi (2) = 19.844, p <0.0001), suggesting that CCV may contribute to diarrhoea in many juvenile dogs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 05:38:15