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Titolo:
PCR detection of bovine papilloma virus DNA in superficial swabs and scrapings from equine sarcoids
Autore:
Martens, A; De Moor, A; Ducatelle, R;
Indirizzi:
State Univ Ghent, Fac Med Vet, Dept Large Anim Surg & Anaesthesiol, B-9820Merelbeke, Belgium State Univ Ghent Merelbeke Belgium B-9820 siol, B-9820Merelbeke, Belgium State Univ Ghent, Fac Med Vet, Dept Pathol Bacteriol & Poultry Dis, B-9820Merelbeke, Belgium State Univ Ghent Merelbeke Belgium B-9820 Dis, B-9820Merelbeke, Belgium
Titolo Testata:
VETERINARY JOURNAL
fascicolo: 3, volume: 161, anno: 2001,
pagine: 280 - 286
SICI:
1090-0233(200105)161:3<280:PDOBPV>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; TUMORS; SEQUENCES; DIAGNOSIS; HORSES;
Keywords:
equine sarcoid; BPV; swab; scraping; diagnosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Martens, A State Univ Ghent, Fac Med Vet, Dept Large Anim Surg & Anaesthesiol, Salisburylaan 133, B-9820 Merelbeke, Belgium State Univ Ghent Salisburylaan 133 Merelbeke Belgium B-9820 um
Citazione:
A. Martens et al., "PCR detection of bovine papilloma virus DNA in superficial swabs and scrapings from equine sarcoids", VET J, 161(3), 2001, pp. 280-286

Abstract

The purpose of this study was to examine if bovine papilloma virus (BPV) DNA can be detected in superficial swabs or scrapings from equine sarcoids. Samples were obtained from 92 sarcoids and 20 non-sarcoidal control lesions. The polymerase chain reaction technique was used with a first primer set to check whether DNA extraction was successful, and with a second primer set specific for BPV-DNA. DNA isolation was successful in 88% of the swabs and 93% of the scrapings. All control lesions were negative for BPV-DNA. BPV-DNA was detected in 88% and 91% of the successful swabs and scrapings from sarcoids respectively. The overall diagnostic sensitivity of the technique was significantly lower compared to the clinical diagnosis of sarcoidsand is unsuitable for occult sarcoids. However, the detection of BPV-DNA in all samples obtained from sarcoids with an ulcerated surface opens new perspectives for the detection of sarcoid involvement in wounds or recurrenceafter sarcoid removal. (C) 2001 Harcourt Publishers Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:03:10