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Titolo:
Endogenous glutathione and platelet function in platelet concentrates stored in plasma or platelet additive solution
Autore:
Hervig, T; Mansoor, MA; Farstad, M;
Indirizzi:
Haukeland Univ Hosp, Blood Bank, N-5021 Bergen, Norway Haukeland Univ Hosp Bergen Norway N-5021 ood Bank, N-5021 Bergen, Norway Cent Hosp Rogaland, Dept Clin Chem, Stavanger, Norway Cent Hosp Rogaland Stavanger Norway , Dept Clin Chem, Stavanger, Norway Haukeland Univ Hosp, Dept Clin Biochem, N-5021 Bergen, Norway Haukeland Univ Hosp Bergen Norway N-5021 Biochem, N-5021 Bergen, Norway
Titolo Testata:
TRANSFUSION MEDICINE
fascicolo: 2, volume: 11, anno: 2001,
pagine: 97 - 104
SICI:
0958-7578(200104)11:2<97:EGAPFI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
REDUCED GLUTATHIONE; BLOOD-PLATELETS; SEROTONIN; METABOLISM; INTEGRITY; STORAGE;
Keywords:
buthionine sulfoximine; cysteamine; endogenous serotonin; glutathione; platelet concentrates; platelet; serotonin secretion; storage; swirling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Hervig, T Haukeland Univ Hosp, Blood Bank, N-5021 Bergen, Norway HaukelandUniv Hosp Bergen Norway N-5021 N-5021 Bergen, Norway
Citazione:
T. Hervig et al., "Endogenous glutathione and platelet function in platelet concentrates stored in plasma or platelet additive solution", TRANSFUS M, 11(2), 2001, pp. 97-104

Abstract

Platelet function was studied in platelet concentrates by assay of the thrombin-induced release of endogenous serotonin and presence of the swirling phenomenon in relation to endogenous glutathione (GSH) and cysteine. In platelets stored in plasma, addition of cysteamine resulted in only a moderate fall in GSH after 5 days of storage, from an average of 14.91 to 11.46 nmol per 10(9) platelets. Exogenously added GSH had no effect, and addition of buthionine sulfoximine (BSO) resulted in almost complete depletion of GSH, to an average of 0.65 nmol per 109 platelets. Addition of cysteamine or GSH resulted in increased endogenous cysteine whereas BSO had no effect. In platelets stored in a platelet additive solution (T-sol(R)), complete depletion of GSH was found in the presence of cysteamine, GSH and BSO. Endogenous serotonin was unchanged during storage both in plasma and in additive solution (2.8 nmol per 10.9 platelets). Despite almost total depletion of endogenous GSH, the thrombin-induced release of serotonin after 5 days' storage was significantly affected only in the presence of BSO in platelets stored in additive solution (mean values 72.3% vs. 63.3% of endogeneousserotonin, P < 0.05). Similarly, addition of cysteamine or GSH had no significant effect on swirling but BSO reduced the swirling score after 5 days' storage in platelet additive solution compared with plasma. After 10 days' storage, there was a significant reduction in swirling in the concentrates where BSO was added (P < 0.05).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 20:55:59