Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Patients with neoplastic and nonneoplastic hematologic diseases acquire CTLA-4 antibodies after blood transfusion
Autore:
Pistillo, MP; Tazzari, PL; Gaudiano, C; Cilla, V; Kato, T; Matsui, T; Nishioka, K; Capanni, P; Conte, R; Ferrara, GB;
Indirizzi:
Natl Canc Inst, Immunogenet Lab, Genoa, Italy Natl Canc Inst Genoa Italy atl Canc Inst, Immunogenet Lab, Genoa, Italy Policlin S Orsola Malpighi, Serv Transfus Med, Bologna, Italy Policlin S Orsola Malpighi Bologna Italy v Transfus Med, Bologna, Italy Matera Hosp, Microcytem Unit, Matera, Italy Matera Hosp Matera ItalyMatera Hosp, Microcytem Unit, Matera, Italy St Marianna Univ, Sch Med, Rheumatol Immunol & Genet Program, Inst Med Sci, Kawasaki, Kanagawa, Japan St Marianna Univ Kawasaki Kanagawa Japan Sci, Kawasaki, Kanagawa, Japan Thalassem Day Hosp, Microcytem Unit, Matera, Italy Thalassem Day Hosp Matera Italy ay Hosp, Microcytem Unit, Matera, Italy Ctr Biotechnol Avanzate, Serv Immunogenet, Dept Oncol Biol & Genet, I-16132 Genoa, Italy Ctr Biotechnol Avanzate Genoa Italy I-16132 Genet, I-16132 Genoa, Italy
Titolo Testata:
TRANSFUSION
fascicolo: 4, volume: 41, anno: 2001,
pagine: 462 - 469
SICI:
0041-1132(200104)41:4<462:PWNANH>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; B-CELL ACTIVATION; NEGATIVE REGULATOR; AUTOIMMUNE-DISEASE; AUTOANTIBODIES; CARCINOMA; BLOCKADE; RESPONSES; APOPTOSIS; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Pistillo, MP Ctr Biotechnol Avanzate, Serv Immunogenet, Dept Oncol Biol & Genet, Largo Rosanna Benzi 10, I-16132 Genoa, Italy Ctr Biotechnol AvanzateLargo Rosanna Benzi 10 Genoa Italy I-16132
Citazione:
M.P. Pistillo et al., "Patients with neoplastic and nonneoplastic hematologic diseases acquire CTLA-4 antibodies after blood transfusion", TRANSFUSION, 41(4), 2001, pp. 462-469

Abstract

BACKGROUND: The presence of antibodies to CTLA-4, a negative regulator of T-cell activation, was investigated in multiply transfused patients with malignant and nonmalignant hematologic diseases. A previous study showed that, in multiply transfused patients, an immune response against nuclear matrix proteins can be induced by WBCs undergoing apoptosis during RBC unit storage. This study evaluated whether the same phenomenon could be involved in the induction of CTLA-4 antibodies in the patients analyzed. STUDY DESIGN AND METHODS: Patient sera were tested for binding to the recombinant full-length CTLA-4 P-galactosidase fusion protein by an ELISA. Immunofluorescence stainings were performed to analyze the CTLA-4 epitopes recognized by the antibodies and to detect such epitopes in the apoptotic cellspresent in the RBC units. RESULTS: CTLA-4 antibodies were found in multiply transfused patients withP-thalassemia (40%) and with other hemolytic diseases (33%) including leukemias (42%). A higher incidence of CTLA-4 antibodies was found in patients receiving non-WBC-reduced blood (88%) than in those receiving WBC-reduced blood (26%). Immunofluorescence staining showed that WBCs undergoing apoptosis in the RBC unit expressed CTLA-4 epitopes. CONCLUSIONS: The apoptotic WBCs present in the RBC units, after cold storage, express CTLA-4 epitopes. These epitopes can be released and induce formation of CTLA-4 antibodies with profound implications in the development ofautoimmune disorders and in facilitating tumor dissemination and metastasis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 13:18:41