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Titolo:
A chemically modified carbon paste electrode with D-lactate dehydrogenase and alanine aminotranferase enzyme sequences for D-lactic acid analysis
Autore:
Shu, HC; Wu, NP;
Indirizzi:
Natl Dong Hwa Univ, Dept Chem, Hualien, Taiwan Natl Dong Hwa Univ Hualien Taiwan Hwa Univ, Dept Chem, Hualien, Taiwan
Titolo Testata:
TALANTA
fascicolo: 2, volume: 54, anno: 2001,
pagine: 361 - 368
SICI:
0039-9140(20010412)54:2<361:ACMCPE>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEQUENTIAL INJECTION-ANALYSIS; FERMENTATION PROCESS; MEDIATOR; POLYMER; NAD(+);
Keywords:
D-lactic acid; D-lactate dehydrogenase; L-alanine aminotransferase; carbon paste electrode; immobilized enzyme reactor; biosensor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Shu, HC Natl Dong Hwa Univ, Dept Chem, Hualien, Taiwan Natl Dong Hwa UnivHualien Taiwan v, Dept Chem, Hualien, Taiwan
Citazione:
H.C. Shu e N.P. Wu, "A chemically modified carbon paste electrode with D-lactate dehydrogenase and alanine aminotranferase enzyme sequences for D-lactic acid analysis", TALANTA, 54(2), 2001, pp. 361-368

Abstract

An amperometric biosensor was constructed for the analysis of D-lactic acid based on immobilizing D-lactate dehydrogenase(D-LDH), alanine aminotransferase (ALT), NAD(+), a redox polymer and polyethylenimine in carbon paste. The effect of addition of ALT in the paste, using enzyme sequences of ALT/D-LDH, was insignificant for D-lactic acid analysis. The responses of D-lactic acid in ALT/D-LDH paste electrode are the same as those in D-LDH paste electrode. However, the interference effect of pyruvate in the sample can besubstantially reduced if sodium glutamate was applied in the carrier solution. When ALT immobilized in control porous glass as an immobilized enzyme reactor (IMER) was mounted in flow injection analysis system with the D-LDHpaste electrode as detector for D-lactate analysis, the interference of the pyruvate can be significantly eliminated. The adverse effect of pyruvate in the samples for D-lactic acid analysis was reduced more effectively in ALT IMER with D-LDH electrode than in ALT;D-LDH electrode. (C) 2001 ElsevierScience B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:56:23