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Titolo:
Experimental attempts to extend the current preimplantation genetic diagnosis with individual karyotypization of human blastomeres
Autore:
Hlinka, D; Dudas, M; Herman, M; Kalina, I;
Indirizzi:
Safarik Univ, Dept Med Biol, Kosice 04066, Slovakia Safarik Univ Kosice Slovakia 04066 Dept Med Biol, Kosice 04066, Slovakia L Pasteur Univ Hosp, Assisted Reprod Ctr, Kosice, Slovakia L Pasteur Univ Hosp Kosice Slovakia sisted Reprod Ctr, Kosice, Slovakia
Titolo Testata:
REPRODUCTION NUTRITION DEVELOPMENT
fascicolo: 1, volume: 41, anno: 2001,
pagine: 91 - 106
SICI:
0926-5287(200101/02)41:1<91:EATETC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; PREMATURE CHROMOSOME CONDENSATION; OKADAIC ACID; MOUSE OOCYTES; PROTEIN PHOSPHATASES; EMBRYOS; CELLS; PHOSPHORYLATION; ABERRATIONS; ACTIVATION;
Keywords:
assisted reproduction; blastomere biopsy; chromosome aberration; fluorescent in situ hybridization (FISH); G-banding; human early embryo; karyotype; okadaic acid; preimplantation genetic diagnosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Dudas, M Safarik Univ, Dept Med Biol, Trieda SNP 1, Kosice 04066, SlovakiaSafarik Univ Trieda SNP 1 Kosice Slovakia 04066 04066, Slovakia
Citazione:
D. Hlinka et al., "Experimental attempts to extend the current preimplantation genetic diagnosis with individual karyotypization of human blastomeres", REPROD NUTR, 41(1), 2001, pp. 91-106

Abstract

The drug-induced chromosome condensation using okadaic acid, a potent protein phosphatase inhibitor, was studied in day 1 to day 4 (D1-D4) spare human preimplantation embryos. In order to obtain cells for genetic tests, we developed a modified displacement blastomere biopsy method. During the okadaic acid treatment, approximately 40% of biopsied cells were lost due to heavy changes of the plasma membrane; this detrimental effect of okadaic acid differed markedly with the respect to the age of embryos. In comparison with the natural embryonic mitotic index, day 1 and day 2 embryonic cells gaveincreased yields of chromosome spreads (up to 51% of the initial D1-D2 cell number); on days 3 and 4 we were not able to obtain from surviving cells more than 31% blastomeres with condensed chromosomes (9% of total D3-D4 cell number). All chromosome spreads were successfully used for recycling in G-banding and subsequent FISH analysis.

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Documento generato il 02/04/20 alle ore 09:57:22