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Titolo:
Simultaneous analysis of clozapine, clomipramine and their metabolites by reversed-phase liquid chromatography
Autore:
Palego, L; DellOsso, L; Marazziti, D; Biondi, L; Sarno, N; Ciapparelli, A; Giromella, A; Giannaccini, G; Lucacchini, A; Cassano, GB;
Indirizzi:
Univ Pisa, Dipartimento Psichiatria Neurobiol Farmacol & Bio, I-56100 Pisa, Italy Univ Pisa Pisa Italy I-56100 urobiol Farmacol & Bio, I-56100 Pisa, Italy
Titolo Testata:
PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY
fascicolo: 3, volume: 25, anno: 2001,
pagine: 519 - 533
SICI:
0278-5846(200104)25:3<519:SAOCCA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
OBSESSIVE-COMPULSIVE DISORDER; N-DESMETHYLCLOMIPRAMINE; SCHIZOPHRENIC-PATIENTS; ULTRAVIOLET DETECTION; PLASMA; SERUM; NORCLOZAPINE; IMIPRAMINE; SYMPTOMS;
Keywords:
clomipramine; clozapine; their demethylated metabolites; dibenzepine; reversed-phase liquid chromatography;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Palego, L Univ Pisa, Dipartimento Psichiatria Neurobiol Farmacol & Bio, Via Bonanno 6, I-56100 Pisa, Italy Univ Pisa Via Bonanno 6 Pisa Italy I-56100 I-56100 Pisa, Italy
Citazione:
L. Palego et al., "Simultaneous analysis of clozapine, clomipramine and their metabolites by reversed-phase liquid chromatography", PROG NEUR-P, 25(3), 2001, pp. 519-533

Abstract

1. The authors present here a sensitive and rapid reversed-phase liquid chromatographic method which enables the simultaneous analysis in plasma of two different drugs and their metabolites: the atypical neuroleptic clozapine and the tricyclic antidepressant clomipramine.2. Samples and the internal standard (dibenzepine) were extracted through an automated solid-phase procedure, evaporated to dryness and injected intothe chromatograph. Mobile phase was a mixture of water and acetonitrile (63:37, v:v) containing TEMED and triethylamine, The total chromatographic time was of 14 min and analyte peaks were detected by means of an ultravioletspectrophotometer preset at 254 nm.3. Results revealed an assay sensitivity of 5 mug/L for clozapine or norclozapine and of 10 mug/L for clomipramine and desmethylclomipramine. Recoveries for these drugs and their metabolites were more than 60% and their coefficient of variation (within day and day-to-day) ranged from 1.3 % to 2.5 %. In spiked plasma, within day and day-to-day coefficients of variability (CV) were less than 5%. The simultaneous evaluation of these two drugs with adequate sensitivity and precision makes it particularly useful for therapeutic drug monitoring during mono- or polypharmacotherapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/11/19 alle ore 02:26:14