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Titolo:
Molecular phylogeny of the genus Kalanchoe (Crassulaceae) inferred from nucleotide sequences of the ITS-1 and ITS-2 regions
Autore:
Gehrig, H; Gaussmann, O; Marx, H; Schwarzott, D; Kluge, M;
Indirizzi:
Smithsonian Trop Res Inst, Unit 0948, APO 340020948, AA, Panama Smithsonian Trop Res Inst APO AA Panama 340020948 O 340020948, AA, Panama Darmstadt Univ Technol, D-64287 Darmstadt, Germany Darmstadt Univ TechnolDarmstadt Germany D-64287 4287 Darmstadt, Germany
Titolo Testata:
PLANT SCIENCE
fascicolo: 5, volume: 160, anno: 2001,
pagine: 827 - 835
SICI:
0168-9452(200104)160:5<827:MPOTGK>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID METABOLISM; DNA POLYMORPHISMS; MADAGASCAR;
Keywords:
Crassulacean acid metabolism (CAM); ecophysiology; Internal Transcribed Spacer (ITS); Kalanchoe; Madagascar; molecular phylogeny;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Gehrig, H Smithsonian Trop Res Inst, Unit 0948, APO 340020948, AA, Panama Smithsonian Trop Res Inst APO AA Panama 340020948 8, AA, Panama
Citazione:
H. Gehrig et al., "Molecular phylogeny of the genus Kalanchoe (Crassulaceae) inferred from nucleotide sequences of the ITS-1 and ITS-2 regions", PLANT SCI, 160(5), 2001, pp. 827-835

Abstract

The study presents an analysis of genotypic diversity in the genus Kalanchoe (Crassulaceae) on the level of Internal Transcribed Spacer (ITS) sequences and the attempt to correlate this diversity with previous findings on ecophysiological behavior, habitat preference. infrageneric taxonomic position of the species and DNA polymorphism derived from RAPD-PCR data. The Kalanchoe species are mainly abundant in Madagascar and eastern continental Africa and perform in situ diverse modes of crassulacean acid metabolism (CAM),an ecophysiologically relevant adaptation of photosynthesis. Total DNA wasextracted from 68 kalanchoe species and varieties. The ITS-1 and ITS-2 regions of the nuclear RNA genes were amplified by polymerase chain reaction, cloned and sequenced. The alignments of the sequences were evaluated by distance (neighbor joining) and character state (maximum parsimony) methods. The main topologies of the obtained ITS phylogenetic trees were quite similar irrespective of the mode of evaluation and show: (A) within the Crassulaceae the genus Kalanchoe forms a monophyletic clade: and (B) within the genus the species form three main clusters which coincide well with the previously reported three infrageneric sections of the species distinguishable by classical taxonomic criteria, the mode of in situ CAM performance, and DNA fragment pattern obtained by RAPD-PCR analyses. Moreover, the ITS phylogenetic trees show that all African Kalanchoe species form a distinct group within the most derived of the three main clusters. This is consistent with the view that the center of phylogenetic radiation of the genus is located inMadagascar from where the species have spread into the continental Africa. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/08/20 alle ore 21:04:18