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Titolo:
Expression and activity of cell-wall-degrading enzymes in the latex of opium poppy, Papaver somniferum L.
Autore:
Pilatzke-Wunderlich, I; Nessler, CL;
Indirizzi:
Virginia Polytech Inst & State Univ, Dept Plant Pathol Physiol & Weed Sci,Blacksburg, VA 24061 USA Virginia Polytech Inst & State Univ Blacksburg VAUSA 24061 VA 24061 USA Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA Texas A&M Univ College Stn TX USA 77843 t Biol, College Stn, TX 77843 USA
Titolo Testata:
PLANT MOLECULAR BIOLOGY
fascicolo: 5, volume: 45, anno: 2001,
pagine: 567 - 576
SICI:
0167-4412(200103)45:5<567:EAAOCE>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
PECTATE LYASE; XYLOGLUCAN ENDOTRANSGLYCOSYLASE; MOLECULAR CHARACTERIZATION; PECTIN METHYLESTERASE; HEVEA-BRASILIENSIS; GENE; PROTEIN; TOMATO; BETA-1,3-GLUCANASE; CELLULASE;
Keywords:
cell wall degradation; opium; Papaver somnferum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Nessler, CL Virginia Polytech Inst & State Univ, Dept Plant Pathol Physiol& Weed Sci,413 Price Hall, Blacksburg, VA 24061 USA Virginia Polytech Inst& State Univ 413 Price Hall Blacksburg VA USA 24061
Citazione:
I. Pilatzke-Wunderlich e C.L. Nessler, "Expression and activity of cell-wall-degrading enzymes in the latex of opium poppy, Papaver somniferum L.", PLANT MOL B, 45(5), 2001, pp. 567-576

Abstract

The alkaloid-rich latex of the opium poppy, Papaver somniferum L., is valued as a source of pharmaceuticals including thebaine, codeine, and morphine, but is also harvested for heroin production. The poppy laticifer system develops through the gradual disappearance of the common walls between differentiating laticifer elements throughout the plant. Gene homologues for cell-wall-degrading enzymes were found during random sequencing of an opium poppy latex cDNA library. RNA gel blot analysis of cellulase, polygalacturonase beta -subunit, 1,3-beta -glucanase, and xyloglucan endotransglycosylase homologues showed their expression was not limited to laticifers. In contrast, poppy gene homologues to pectin methylesterase (PME), pectin acetylesterase (PAE) and pectate lyase (PL) where all highly expressed and latex-specific. Enzyme assays confirmed the presence of PME, PAE, and PL activities in latex serum. The abundance of transcripts encoding pectin-degrading enzymes in latex suggests that these enzymes may play an important role in laticifer development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:28:24