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Titolo:
Expression of tumor necrosis factor-alpha, interleukin-6, and interferon-gamma in spontaneous chronic pancreatitis in the WBN/Kob rat
Autore:
Xie, MJ; Motoo, Y; Su, SB; Sawabu, N;
Indirizzi:
Kanazawa Univ, Canc Res Inst, Dept Internal Med & Med Oncol, Kanazawa, Ishikawa 9218044, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9218044 wa, Ishikawa 9218044, Japan
Titolo Testata:
PANCREAS
fascicolo: 4, volume: 22, anno: 2001,
pagine: 400 - 408
SICI:
0885-3177(200105)22:4<400:EOTNFI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA EXPRESSION; GROWTH-FACTOR-BETA; IFN-GAMMA; GENE-EXPRESSION; STELLATE CELLS; TNF-ALPHA; CYTOKINE PRODUCTION; DIABETIC STRAIN; LIVER FIBROSIS; ITO CELLS;
Keywords:
TNF-alpha; IL-6; IFN-gamma; chronic pancreatitis; WBN/Kob rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Motoo, Y Kanazawa Univ, Canc Res Inst, Dept Internal Med & Med Oncol, 4-86Yoneizumi, Kanazawa, Ishikawa 9218044, Japan Kanazawa Univ 4-86 Yoneizumi Kanazawa Ishikawa Japan 9218044 apan
Citazione:
M.J. Xie et al., "Expression of tumor necrosis factor-alpha, interleukin-6, and interferon-gamma in spontaneous chronic pancreatitis in the WBN/Kob rat", PANCREAS, 22(4), 2001, pp. 400-408

Abstract

To clarify the pathophysiological significance of cytokines in chronic pancreatitis (CP), we analyzed tissue expressions of various cytokines in the onset and progression of spontaneous CP in the WBN/Kob rat. Four-week-old male WBN/Kob rats were fed a special pellet diet (MB-3) for 20 weeks, and 6 rats were killed every 4 weeks. Pathologically, CP occurred at 12 weeks andprogressed thereafter. The inflammation and fibrosis peaked at 12 and 16 weeks, respectively. By semiquantitative reverse transcription-polymerase chain reaction, the expression of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and interferon (IFN)-gamma mRNAs peaked at 8, 12, and 16 weeks,respectively. Immunohistochemistry showed IL-6 expression in infiltrating inflammatory cells and Vascular endothelial cells, whereas TNF-alpha. was expressed in both acinar and infiltrating cells. IFN-gamma was localized to acinar, infiltrating and ductal cells, and its expression intensity showed significant correlation with those of fibrosis, type ill collagen and a-smooth muscle actin. The in situ hybridization results were consistent with the RT-PCR data. These results suggest that tissue expressions of TNF-alpha and IL-6 are involved in the onset of pancreatitis and that TFN-gamma expression is related to the progression of CP.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 09:34:19