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Titolo:
6-hydroxydopamine increases hydroxyl free radical production and DNA damage in rat striatum
Autore:
Ferger, B; Rose, S; Jenner, A; Halliwell, B; Jenner, P;
Indirizzi:
Univ London Kings Coll, Guys Kings & St Thomas Sch Biomed Sci, Wolfson CtrAge Related Dis, London SE1 1UL, England Univ London Kings Coll London England SE1 1UL s, London SE1 1UL, England Univ London Kings Coll, Dept Pharm, Biochem Toxicol Grp, London SE1 8WA, England Univ London Kings Coll London England SE1 8WA p, London SE1 8WA, England Natl Univ Singapore, Dept Biochem, Singapore 119260, Singapore Natl Univ Singapore Singapore Singapore 119260 ngapore 119260, Singapore
Titolo Testata:
NEUROREPORT
fascicolo: 6, volume: 12, anno: 2001,
pagine: 1155 - 1159
SICI:
0959-4965(20010508)12:6<1155:6IHFRP>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PARKINSONS-DISEASE; SUBSTANTIA-NIGRA; L-DOPA; BRAIN; CHROMATOGRAPHY; GENERATION; ACTIVATION; ADDUCTS; SYSTEM;
Keywords:
DNA base damage; 6-hydroxydopamine; microdialysis; Parkinson's disease; reactive oxygen species; salicylate assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Ferger, B Swiss Fed Inst Technol, Behav Neurobiol Lab, Schorenstr 16, CH-8603 Schwerzenbach, Switzerland Swiss Fed Inst Technol Schorenstr 16 Schwerzenbach Switzerland CH-8603
Citazione:
B. Ferger et al., "6-hydroxydopamine increases hydroxyl free radical production and DNA damage in rat striatum", NEUROREPORT, 12(6), 2001, pp. 1155-1159

Abstract

Oxidative damage is considered to be an important factor of 6-hydroxydopamine (6-OHDA) toxicity. To address this issue, microdialysis probes were implanted into the striatum of Wistar rats and perfused with 6-OHDA. Salicylate was included in the perfusion fluid to measure 2,3-dihydroxybenzoic acid (2,3-DHBA) as a marker of hydroxyl radical formation using HPLC with electrochemical detection. Additionally, striatal tissue was analysed for DNA base alterations using gas chromatography-mass spectrometry. 6-OHDA administration resulted in a rapid and substantial 6.6-fold increase in 2,3-DHBA formation and also increased levels of the modified DNA bases 5-hydroxycytosine, hypoxanthine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine. Hydroxyl radical formation and DNA base alterations are early phenomena of 6-OHDA toxicity and provide clues to the processes that may be involved in the initiation of cell death in Parkinson's disease. NeuroReport 12:1155-1159 (C) 2001Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 07:29:16