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Titolo:
Melatonin acts as antioxidant and prooxidant in an organotypic slice culture model of Alzheimer's disease
Autore:
Clapp-Lilly, KL; Smith, MA; Perry, G; Harris, PL; Zhu, XW; Duffy, LK;
Indirizzi:
Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK 99775 USA Univ Alaska Fairbanks Fairbanks AK USA 99775 iol, Fairbanks, AK 99775 USA Univ Alaska Fairbanks, Dept Chem, Fairbanks, AK 99775 USA Univ Alaska Fairbanks Fairbanks AK USA 99775 hem, Fairbanks, AK 99775 USA Case Western Reserve Univ, Inst Pathol, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA
Titolo Testata:
NEUROREPORT
fascicolo: 6, volume: 12, anno: 2001,
pagine: 1277 - 1280
SICI:
0959-4965(20010508)12:6<1277:MAAAAP>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDATIVE STRESS; BETA; PROTEIN; PC12;
Keywords:
Alzheimer's disease; amyloid; low oxygen partial pressure; melatonin; organotypic culture; pro-oxidant;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Duffy, LK Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK 99775 USAUniv Alaska Fairbanks Fairbanks AK USA 99775 anks, AK 99775 USA
Citazione:
K.L. Clapp-Lilly et al., "Melatonin acts as antioxidant and prooxidant in an organotypic slice culture model of Alzheimer's disease", NEUROREPORT, 12(6), 2001, pp. 1277-1280

Abstract

An organotypic mouse brain slice culture system of Alzheimer's disease (AD) under low oxygen partial pressures was developed to determine the antioxidant properties of the pineal hormone melatonin in vitro. Assays for biochemical markers of oxidative stress including redox active iron assay, heme-oxygenase-1 and 8-hydroxyguanosine immunoreactivity were performed along with morphological analysis for stressed tissue following amyloid-beta (A beta) 1-40 insult. Melatonin (100 muM) significantly reduced the appearance of condensed chromatin, redox active iron, heme-oxygenase-1 induction and 8-hydroxyguanosine inmunoreactivity caused by 50 muM A beta. Melatonin also prevented A beta -induced morphological signs of oxidative stress in tissue ultrastucture, including edema and dark degenerating profiles as visualized under electron microscope. At elevated concentrations (1 mM), melatonin induced redox active iron and heme-oxgenase-1 immunoreactivity. Thus, while melatonin may be a potential therapeutic agent in the prevention of oxidative stress associated with A beta and AD, it can also induce markers of oxidative stress at millimolar concentrations. NeuroReport 12:1277-1280 (C) 2001 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 11:26:06