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Titolo:
A helical lid converts a sulfotransferase to a dehydratase
Autore:
Pakhomova, S; Kobayashi, M; Buck, J; Newcomer, ME;
Indirizzi:
Vanderbilt Univ, Sch Med, Dept Biochem, Nashville, TN 37232 USA VanderbiltUniv Nashville TN USA 37232 t Biochem, Nashville, TN 37232 USA Cornell Univ, Weill Med Coll, Dept Pharmacol, New York, NY 10021 USA Cornell Univ New York NY USA 10021 Dept Pharmacol, New York, NY 10021 USA
Titolo Testata:
NATURE STRUCTURAL BIOLOGY
fascicolo: 5, volume: 8, anno: 2001,
pagine: 447 - 451
SICI:
1072-8368(200105)8:5<447:AHLCAS>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIGAND-BINDING DOMAIN; CRYSTAL-STRUCTURE; ESTROGEN SULFOTRANSFERASE; RETINOL DEHYDRATASE; SUBSTRATE-SPECIFICITY; SPODOPTERA-FRUGIPERDA; MOLECULAR-BIOLOGY; ACID; PURIFICATION; MECHANISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Newcomer, ME Vanderbilt Univ, Sch Med, Dept Biochem, 221 Kirkland Hall, Nashville, TN 37232 USA Vanderbilt Univ 221 Kirkland Hall Nashville TN USA 37232 USA
Citazione:
S. Pakhomova et al., "A helical lid converts a sulfotransferase to a dehydratase", NAT ST BIOL, 8(5), 2001, pp. 447-451

Abstract

We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase.

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Documento generato il 30/03/20 alle ore 00:53:34