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Titolo:
Expression of rhodopsin and arrestin during the light-dark cycle in Drosophila
Autore:
Hartman, SJ; Menon, I; Haug-Collet, K; Colley, NJ;
Indirizzi:
Univ Wisconsin, Dept Ophthalmol & Visual Sci, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 l & Visual Sci, Madison, WI 53706 USA Univ Wisconsin, Dept Genet, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 in, Dept Genet, Madison, WI 53706 USA
Titolo Testata:
MOLECULAR VISION
fascicolo: 14, volume: 7, anno: 2001,
pagine: 95 - 100
SICI:
1090-0535(20010417)7:14<95:EORAAD>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLOPHILIN HOMOLOG NINAA; ROD OUTER SEGMENT; STATIONARY NIGHT BLINDNESS; MESSENGER-RNA; PHOTORECEPTOR MEMBRANE; RETINAL DEGENERATION; RETINITIS-PIGMENTOSA; MUTANT DROSOPHILA; GENE-EXPRESSION; OGUCHI-DISEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Colley, NJ Univ Wisconsin, Dept Ophthalmol & Visual Sci, 600 Highland Ave,K6-460 CSC,Madison, WI 53706 USA Univ Wisconsin 600 Highland Ave,K6-460 CSCMadison WI USA 53706
Citazione:
S.J. Hartman et al., "Expression of rhodopsin and arrestin during the light-dark cycle in Drosophila", MOL VIS, 7(14), 2001, pp. 95-100

Abstract

Purpose: To determine the protein and transcript levels for rhodopsin (Rh1), arrestin 1 (Arr1), and arrestin 2 (Arr2) over a 12 h light/12 h dark cycle in the retina of the fruit fly, Drosophila melanogaster. This information is important for understanding the process of photoreceptor membrane turnover. Methods: Drosophila were entrained for several generations to a daily 12 hlight/12 h dark cycle. They were sacrificed at 4 h intervals, beginning atthe time of onset of the light phase. Proteins were resolved by polyacrylamide gel electrophoresis (PAGE) and subjected to immunoblot analysis using antibodies directed to rhodopsin, NinaA, Arr1, and Arr2. Northern blots were incubated with riboprobes corresponding to the rhodopsin gene (ninaE), arrestin1 (arr1), and arrestin2 (arr2). Results: In entrained Drosophila, protein and mRNA levels for rhodopsin, arrestin1, and arrestin2 were constant during a 12 h light/12 h dark cycle. Conclusions: These results indicate that rhodopsin and arrestin protein synthesis in Drosophila photoreceptors do not fluctuate on a daily cycle. These findings are similar to those obtained in Xenopus laevis, but in contrast to a variety of other vertebrate and invertebrate species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 03:25:55